Hepatoprotective Effect of Lactobacillus plantarum HFY09 on Ethanol-Induced Liver Injury in Mice
- 2021-06-24
- Frontiers in Nutrition 8
- Y. Gan
- J. Tong
- Xianrong Zhou
- X. Long
- Yanni Pan
- Weiwei Liu
- Xin Zhao
- PubMed: 34249992
- DOI: 10.3389/fnut.2021.684588
Abstract
Lactobacillus plantarum is a bacterial strain that is used as a probiotic with health-promoting effects. Our study investigated the hepatoprotective effect of Lactobacillus plantarum HFY09 (LP-HFY09) in mice with ethanol-induced liver injury. The protection afforded by LP-HFY09 was evaluated by observing the morphology of hepatic tissue and measuring liver lipid indexes and function indexes, levels of anti-oxidative enzymes, and anti-inebriation enzymes, as well as oxidative metabolism-related gene expression. Gavage administration of LP-HFY09 [1 × 109 CFU/kg body weight (bw)] limited the loss of bw, alcohol damage to the liver, and maintained the normal hepatic tissue morphology. Lactobacillus plantarum HFY09 intervention in ethanol-induced mice led to decreases in serum triglyceride (TG), total cholesterol (TC), aspartic transaminase, alanine transaminase, hyaluronidase (HAase), and precollagen III (PC III), and increases in liver alcohol dehydrogenase (ADH), and acetaldehyde dehydrogenase (ALDH). Lactobacillus plantarum HFY09 assisted with alleviating inflammation by elevating the level of interleukin 10 (IL-10) and decreasing the levels of pro-inflammatory factors [IL-6, IL-1β, and tumor necrosis factor-α (TNF)-α]. Lactobacillus plantarum HFY09 significantly elevated hepatic levels of superoxide dismutase (SOD) and glutathione (GSH), and decreased liver malondialdehyde (MDA) from 3.45 to 1.64 nmol/mg protein. Lactobacillus plantarum HFY09 exhibited an overall strong regulatory effect on liver protection when compared to that of commercial Lactobacillus delbrueckii subsp. bulgaricus. The hepatoprotective effect of LP-HFY09 was reflected by the upregulated expression of peroxisome proliferator activated-receptors α, SOD1, SOD2, glutathione peroxidase (GSH-Px), nicotinamide adenine dinucleotide phosphate (NADPH), and catalase (CAT), and the downregulated expression of cyclooxygenase-1 (COX1), c-Jun N-terminal kinase (JNK), and extracellular regulated protein kinases (ERK). Administration of LP-HFY09 at a concentration of 1.0 × 109 CFU/kg bw could be a potential intervention, for people who frequently consume alcohol.
Keywords: alcoholic liver injury; anti-inflammatory; antioxidant; hepatoprotective effect; lactic acid bacteria.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Enhanced Antioxidant Activity | Beneficial | Large |
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Improved Alcohol Metabolism | Beneficial | Large |
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Improved Gene Expression for Liver Protection | Beneficial | Large |
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Improved Liver Function | Beneficial | Large |
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Reduced Inflammation Levels | Beneficial | Large |
| Lactobacillus plantarum DSM 6595 | Improved Antioxidant Levels | Beneficial | Large |
| Lactobacillus plantarum DSM 6595 | Improved Liver Function | Beneficial | Large |
| Lactobacillus plantarum DSM 6595 | Increased Expression of Protective Genes | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6595 | Reduced Expression of Hyphae-specific Genes | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6595 | Reduced Inflammation Levels | Beneficial | Large |
| Lactobacillus plantarum DSM 6596 | Improved Antioxidant Enzyme Levels | Beneficial | Large |
| Lactobacillus plantarum DSM 6596 | Improved Liver Function | Beneficial | Large |
| Lactobacillus plantarum DSM 6596 | Reduced Inflammation Levels | Beneficial | Large |
| Lactobacillus plantarum DSM 6596 | Reduced Lipid Peroxidation | Beneficial | Large |
| Lactobacillus plantarum HEAL9 | Increased Antioxidant Enzyme Levels | Beneficial | Large |
| Lactobacillus plantarum HEAL9 | Increased Liver Alcohol Metabolizing Enzyme Activity | Beneficial | Moderate |
| Lactobacillus plantarum HEAL9 | Reduced Inflammatory Markers | Beneficial | Large |
| Lactobacillus plantarum HEAL9 | Reduced Liver Enzyme Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Elevated Antioxidant Levels | Beneficial | Large |
| Lactobacillus plantarum LP-01 | Improved Enzyme Levels (ADH, ALDH) | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Improved Liver Morphology | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced DSS-Induced Body Weight Loss | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced Inflammation Levels | Beneficial | Large |
| Lactobacillus plantarum LP-01 | Reduced Serum Triglycerides | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced Total Cholesterol Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP09 | Improved Antioxidant Enzyme Levels | Beneficial | Large |
| Lactobacillus plantarum LP09 | Improved Liver Enzyme Function | Beneficial | Large |
| Lactobacillus plantarum LP09 | Reduced Liver Damage | Beneficial | Large |
| Lactobacillus plantarum LP09 | Reduced Pro-Inflammatory Markers | Beneficial | Large |
| Lactobacillus plantarum LP09 | Regulation of Oxidative Stress-Related Gene Expression | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Increased Alcohol Dehydrogenase Levels in Liver | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Increased ALDH Levels | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Increased Glutathione Levels | Beneficial | Large |
| Lactobacillus plantarum Lp-115 | Increased Interleukin-10 Levels | Beneficial | Large |
| Lactobacillus plantarum Lp-115 | Increased Superoxide Dismutase Levels | Beneficial | Large |
| Lactobacillus plantarum Lp-115 | Reduced Alanine Transaminase Activity | Beneficial | Large |
| Lactobacillus plantarum Lp-115 | Reduced Aspartate Transaminase Activity | Beneficial | Large |
| Lactobacillus plantarum Lp-115 | Reduced Hyaluronidase Activity | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Reduced Liver Malondialdehyde Levels | Beneficial | Large |
| Lactobacillus plantarum Lp-115 | Reduced Precollagen III Levels | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Reduced Serum Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum Lp-115 | Reduced Serum Triglyceride Levels | Beneficial | Large |
| Lactobacillus plantarum SD-5209 | Enhanced Antioxidative Enzyme Activity | Beneficial | Large |
| Lactobacillus plantarum SD-5209 | Reduced Inflammatory Markers | Beneficial | Large |
| Lactobacillus plantarum SD-5209 | Reduced Liver Damage Markers Levels | Beneficial | Moderate |
| Lactobacillus plantarum SD-5209 | Reduced Oxidative Stress Markers | Beneficial | Moderate |
| Lactobacillus plantarum SD5209 | Elevated Antioxidant Levels | Beneficial | Large |
| Lactobacillus plantarum SD5209 | Enhanced Gene Expression for Antioxidative Stress | Beneficial | Large |
| Lactobacillus plantarum SD5209 | Improved Liver Function | Beneficial | Large |
| Lactobacillus plantarum SD5209 | Reduced Inflammation Levels | Beneficial | Large |
| Lactobacillus plantarum SD5209 | Reduced Liver Damage | Beneficial | Large |
| Lactobacillus plantarum VPro 10 | Improved Antioxidant Enzyme Levels | Beneficial | Large |
| Lactobacillus plantarum VPro 10 | Improved Liver Enzyme Function | Beneficial | Large |
| Lactobacillus plantarum VPro 10 | Improved Oxidative Metabolism Regulation | Beneficial | Large |
| Lactobacillus plantarum VPro 10 | Limited Weight Loss | Beneficial | Large |
| Lactobacillus plantarum VPro 10 | Reduced Liver Inflammation | Beneficial | Moderate |
| Lactobacillus plantarum VPro 10 | Reduced Total Cholesterol | Beneficial | Moderate |
| Lactobacillus plantarum VPro 10 | Reduced Triglyceride Levels | Beneficial | Moderate |