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Hepatoprotective Effect of Lactobacillus plantarum HFY09 on Ethanol-Induced Liver Injury in Mice

  • 2021-06-24
  • Frontiers in Nutrition 8
    • Y. Gan
    • J. Tong
    • Xianrong Zhou
    • X. Long
    • Yanni Pan
    • Weiwei Liu
    • Xin Zhao

Abstract

Lactobacillus plantarum is a bacterial strain that is used as a probiotic with health-promoting effects. Our study investigated the hepatoprotective effect of Lactobacillus plantarum HFY09 (LP-HFY09) in mice with ethanol-induced liver injury. The protection afforded by LP-HFY09 was evaluated by observing the morphology of hepatic tissue and measuring liver lipid indexes and function indexes, levels of anti-oxidative enzymes, and anti-inebriation enzymes, as well as oxidative metabolism-related gene expression. Gavage administration of LP-HFY09 [1 × 109 CFU/kg body weight (bw)] limited the loss of bw, alcohol damage to the liver, and maintained the normal hepatic tissue morphology. Lactobacillus plantarum HFY09 intervention in ethanol-induced mice led to decreases in serum triglyceride (TG), total cholesterol (TC), aspartic transaminase, alanine transaminase, hyaluronidase (HAase), and precollagen III (PC III), and increases in liver alcohol dehydrogenase (ADH), and acetaldehyde dehydrogenase (ALDH). Lactobacillus plantarum HFY09 assisted with alleviating inflammation by elevating the level of interleukin 10 (IL-10) and decreasing the levels of pro-inflammatory factors [IL-6, IL-1β, and tumor necrosis factor-α (TNF)-α]. Lactobacillus plantarum HFY09 significantly elevated hepatic levels of superoxide dismutase (SOD) and glutathione (GSH), and decreased liver malondialdehyde (MDA) from 3.45 to 1.64 nmol/mg protein. Lactobacillus plantarum HFY09 exhibited an overall strong regulatory effect on liver protection when compared to that of commercial Lactobacillus delbrueckii subsp. bulgaricus. The hepatoprotective effect of LP-HFY09 was reflected by the upregulated expression of peroxisome proliferator activated-receptors α, SOD1, SOD2, glutathione peroxidase (GSH-Px), nicotinamide adenine dinucleotide phosphate (NADPH), and catalase (CAT), and the downregulated expression of cyclooxygenase-1 (COX1), c-Jun N-terminal kinase (JNK), and extracellular regulated protein kinases (ERK). Administration of LP-HFY09 at a concentration of 1.0 × 109 CFU/kg bw could be a potential intervention, for people who frequently consume alcohol.

Keywords: alcoholic liver injury; anti-inflammatory; antioxidant; hepatoprotective effect; lactic acid bacteria.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus Bulgaricus & Lactobacillus PlantarumEnhanced Antioxidant ActivityBeneficial
Large
Lactobacillus Bulgaricus & Lactobacillus PlantarumImproved Alcohol MetabolismBeneficial
Large
Lactobacillus Bulgaricus & Lactobacillus PlantarumImproved Gene Expression for Liver ProtectionBeneficial
Large
Lactobacillus Bulgaricus & Lactobacillus PlantarumImproved Liver FunctionBeneficial
Large
Lactobacillus Bulgaricus & Lactobacillus PlantarumReduced Inflammation LevelsBeneficial
Large
Lactobacillus plantarum DSM 6595Improved Antioxidant LevelsBeneficial
Large
Lactobacillus plantarum DSM 6595Improved Liver FunctionBeneficial
Large
Lactobacillus plantarum DSM 6595Increased Expression of Protective GenesBeneficial
Moderate
Lactobacillus plantarum DSM 6595Reduced Expression of Hyphae-specific GenesBeneficial
Moderate
Lactobacillus plantarum DSM 6595Reduced Inflammation LevelsBeneficial
Large
Lactobacillus plantarum DSM 6596Improved Antioxidant Enzyme LevelsBeneficial
Large
Lactobacillus plantarum DSM 6596Improved Liver FunctionBeneficial
Large
Lactobacillus plantarum DSM 6596Reduced Inflammation LevelsBeneficial
Large
Lactobacillus plantarum DSM 6596Reduced Lipid PeroxidationBeneficial
Large
Lactobacillus plantarum HEAL9Increased Antioxidant Enzyme LevelsBeneficial
Large
Lactobacillus plantarum HEAL9Increased Liver Alcohol Metabolizing Enzyme ActivityBeneficial
Moderate
Lactobacillus plantarum HEAL9Reduced Inflammatory MarkersBeneficial
Large
Lactobacillus plantarum HEAL9Reduced Liver Enzyme LevelsBeneficial
Moderate
Lactobacillus plantarum LP-01Elevated Antioxidant LevelsBeneficial
Large
Lactobacillus plantarum LP-01Improved Enzyme Levels (ADH, ALDH)Beneficial
Moderate
Lactobacillus plantarum LP-01Improved Liver MorphologyBeneficial
Moderate
Lactobacillus plantarum LP-01Reduced DSS-Induced Body Weight LossBeneficial
Moderate
Lactobacillus plantarum LP-01Reduced Inflammation LevelsBeneficial
Large
Lactobacillus plantarum LP-01Reduced Serum TriglyceridesBeneficial
Moderate
Lactobacillus plantarum LP-01Reduced Total Cholesterol LevelsBeneficial
Moderate
Lactobacillus plantarum LP09Improved Antioxidant Enzyme LevelsBeneficial
Large
Lactobacillus plantarum LP09Improved Liver Enzyme FunctionBeneficial
Large
Lactobacillus plantarum LP09Reduced Liver DamageBeneficial
Large
Lactobacillus plantarum LP09Reduced Pro-Inflammatory MarkersBeneficial
Large
Lactobacillus plantarum LP09Regulation of Oxidative Stress-Related Gene ExpressionBeneficial
Moderate
Lactobacillus plantarum Lp-115Increased Alcohol Dehydrogenase Levels in LiverBeneficial
Moderate
Lactobacillus plantarum Lp-115Increased ALDH LevelsBeneficial
Moderate
Lactobacillus plantarum Lp-115Increased Glutathione LevelsBeneficial
Large
Lactobacillus plantarum Lp-115Increased Interleukin-10 LevelsBeneficial
Large
Lactobacillus plantarum Lp-115Increased Superoxide Dismutase LevelsBeneficial
Large
Lactobacillus plantarum Lp-115Reduced Alanine Transaminase ActivityBeneficial
Large
Lactobacillus plantarum Lp-115Reduced Aspartate Transaminase ActivityBeneficial
Large
Lactobacillus plantarum Lp-115Reduced Hyaluronidase ActivityBeneficial
Moderate
Lactobacillus plantarum Lp-115Reduced Liver Malondialdehyde LevelsBeneficial
Large
Lactobacillus plantarum Lp-115Reduced Precollagen III LevelsBeneficial
Moderate
Lactobacillus plantarum Lp-115Reduced Serum Total Cholesterol LevelsBeneficial
Large
Lactobacillus plantarum Lp-115Reduced Serum Triglyceride LevelsBeneficial
Large
Lactobacillus plantarum SD5209Elevated Antioxidant LevelsBeneficial
Large
Lactobacillus plantarum SD5209Enhanced Gene Expression for Antioxidative StressBeneficial
Large
Lactobacillus plantarum SD5209Improved Liver FunctionBeneficial
Large
Lactobacillus plantarum SD5209Reduced Inflammation LevelsBeneficial
Large
Lactobacillus plantarum SD5209Reduced Liver DamageBeneficial
Large
Lactobacillus plantarum SD-5209Enhanced Antioxidative Enzyme ActivityBeneficial
Large
Lactobacillus plantarum SD-5209Reduced Inflammatory MarkersBeneficial
Large
Lactobacillus plantarum SD-5209Reduced Liver Damage Markers LevelsBeneficial
Moderate
Lactobacillus plantarum SD-5209Reduced Oxidative Stress MarkersBeneficial
Moderate
Lactobacillus plantarum VPro 10Improved Antioxidant Enzyme LevelsBeneficial
Large
Lactobacillus plantarum VPro 10Improved Liver Enzyme FunctionBeneficial
Large
Lactobacillus plantarum VPro 10Improved Oxidative Metabolism RegulationBeneficial
Large
Lactobacillus plantarum VPro 10Limited Weight LossBeneficial
Large
Lactobacillus plantarum VPro 10Reduced Liver InflammationBeneficial
Moderate
Lactobacillus plantarum VPro 10Reduced Total CholesterolBeneficial
Moderate
Lactobacillus plantarum VPro 10Reduced Triglyceride LevelsBeneficial
Moderate

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