Skip to main content
Evidence-Based Supplement Research
Evidence-Based Supplement Research
Pillser
Search
Sign UpLog In

Cordyceps sinensis accelerates stem cell recruitment to human skeletal muscle after exercise.

  • 2024
  • Food & function 15(8)
    • Luthfia Dewi
    • Yu-Chieh Liao
    • Wei-Horng Jean
    • Kuo-Chin Huang
    • Chih-Yang Huang
    • Liang-Kung Chen
    • Andrew Nicholls
    • Li-Fan Lai
    • Chia-Hua Kuo

Study Design

Type
Randomized Controlled Trial (RCT)
Population
14 young adults (age: 24 ± 0.8 years)
Methods
A randomized, double-blind, placebo-controlled crossover study. A placebo (1 g cornstarch) and Cordyceps (1 g Cordyceps sinensis) were administered before exercise (at 120% maximal aerobic power). Multiple biopsies were taken from the vastus lateralis for muscle tissue analysis before and after HIIE.
Blinding
Double-blind
  • Rigorous Journal
Cordyceps sinensis is a parasitic fungus known to induce immune responses. The impact of Cordyceps supplementation on stem cell homing and expansion to human skeletal muscle after exercise remains unexplored. In this study, we examined how pre-exercise Cordyceps supplementation influences cell infiltration, CD34+ cell recruitment, and Pax7+ cell expansion in human skeletal muscle after high-intensity interval exercise (HIIE) on a cycloergometer. A randomized, double-blind, placebo-controlled crossover study was conducted with 14 young adults (age: 24 ± 0.8 years). A placebo (1 g cornstarch) and Cordyceps (1 g Cordyceps sinensis) were administered before exercise (at 120% maximal aerobic power). Multiple biopsies were taken from the vastus lateralis for muscle tissue analysis before and after HIIE. This exercise regimen doubled the VEGF mRNA in the muscle at 3 h post-exercise (P = 0.006). A significant necrotic cell infiltration (+284%, P = 0.05) was observed 3 h after HIIE and resolved within 24 h. This response was substantially attenuated by Cordyceps supplementation. Moreover, we observed increases in CD34+ cells at 24 h post-exercise, notably accelerated by Cordyceps supplementation to 3 h (+51%, P = 0.002). This earlier response contributed to a four-fold expansion in Pax7+ cell count, as demonstrated by immunofluorescence double staining (CD34+/Pax7+) (P = 0.01). In conclusion, our results provide the first human evidence demonstrating the accelerated resolution of exercise-induced muscle damage by Cordyceps supplementation. This effect is associated with earlier stem cell recruitment into the damaged sites for muscle regeneration.

Research Insights

  • Cordyceps→Increased CD34+ Cell Recruitment

    increases in CD34+ cells at 24 h post-exercise, notably accelerated by Cordyceps supplementation to 3 h (+51%, P = 0.002).

    Effect
    Beneficial
    Effect size
    Moderate
    Dose
    1 g
  • Cordyceps→Increased Pax7+ Cell Count

    This earlier response contributed to a four-fold expansion in Pax7+ cell count, as demonstrated by immunofluorescence double staining (CD34+/Pax7+) (P = 0.01).

    Effect
    Beneficial
    Effect size
    Large
    Dose
    1 g
  • Cordyceps→Increased VEGF mRNA Level

    This exercise regimen doubled the VEGF mRNA in the muscle at 3 h post-exercise (P = 0.006).

    Effect
    Neutral
    Effect size
    Small
    Dose
    1 g
  • Cordyceps→Reduced Necrotic Cell Infiltration

    A significant necrotic cell infiltration (+284%, P = 0.05) was observed 3 h after HIIE and resolved within 24 h. This response was substantially attenuated by Cordyceps supplementation.

    Effect
    Beneficial
    Effect size
    Large
    Dose
    1 g
Back to top