Expressing Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Single-Chain Antibody Can Inhibit PRRSV Replication and Change the Intestinal Flora Structure of Piglets.

2025-03-03
International journal of molecular sciences 26(5)
- Tianming Niu
- Tianqi Fan
- Yingjie Wang
- Kuipeng Gao
- Jinhui Zhao
- Ruyu Wang
- Xiaolei Chen
- Junhong Xing
- Jingjing Qiu
- Boshi Zou
- Shuhui Fan
- Shi Zhang
- Qiong Wu
- Guilian Yang
- Nan Wang
- Yan Zeng
- Xin Cao
- Yanlong Jiang
- Jianzhong Wang
- Haibin Huang
- Wentao Yang
- Chunwei Shi
- Zhipeng Li
- Chunfeng Wang

PubMed: 40076879
DOI: 10.3390/ijms26052257

Porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that can cause reproductive disorders in sows and affect the breathing of piglets, seriously endangering pig breeding worldwide. In this study, Lactobacillus plantarum NC8 was used as the expression delivery vector of foreign proteins, and a single-chain antibody was designed based on an mAb-PN9cx3 sequence. Three recombinant strains of Lactobacillus plantarum, namely, NC8/pSIP409-pgsA'-PN9cx3-scFV(E), NC8/pSIP409-pgsA'-PN9cx3-HC(E), and NC8/pSIP409-pgsA'-PN9cx3-LC(E), were successfully constructed. In an in vitro test, the viral load of each experimental group was significantly lower than that of the control group (p < 0.01). In the piglet challenge protection test, the percentage of CD3⁺CD8⁺T cells in the blood of piglets given complex lactic acid bacteria was significantly increased before and after the challenge (p < 0.01); the body temperature of piglets in this group was normal, the viral load of each organ was reduced, and the obvious pathological changes in each tissue were alleviated. At the same time, the abundance of Bacteroides, Fusobacterium, and other bacteria in the intestinal tracts of the piglets changed, affecting the metabolism of carbohydrates and amino acids and the differentiation of Th1 and Th2 cells. This experiment provides a feasible strategy and method for the design of a PRRSV vaccine.

Research Insights

Supplement	Health Outcome	Effect Type	Effect Size
Lactobacillus salivarius UCC118	Altered Gut Microbiota Composition	Neutral	Moderate
Lactobacillus salivarius UCC118	Improved Clinical Protection Against Viral Challenge	Beneficial	Large
Lactobacillus salivarius UCC118	Increased CD3+CD8+ Cell Counts	Beneficial	Moderate
Lactobacillus salivarius UCC118	Reduced RSV Replication	Beneficial	Large