Latilactobacillus sakei CNTA 173 Reduces Fat Mass by Modulating Sphingolipid Metabolism in Diet-Induced Obese Wistar Rats.

2025-10-30
Journal of agricultural and food chemistry 73(45)

PubMed: 41166528
DOI: 10.1021/acs.jafc.5c10238

Study Design

Population: diet-induced obese Wistar rats
Methods: investigate the physiological and metabolic effects of Latilactobacillus sakei CNTA 173 (10^9 CFU/day); biochemical, 16S microbiota, untargeted metabolomic, and gene expression analyses were performed, as well as an in vivo toxicological study to demonstrate its safety

Rigorous Journal

The use of probiotics with health-promoting activities has emerged as a strategy to combat specific hallmarks of obesity, such as excessive fat accumulation and associated inflammation. Here, we investigate the physiological and metabolic effects of Latilactobacillus sakei CNTA 173 (10⁹ CFU/day) in diet-induced obese Wistar rats. Biochemical, 16S microbiota, untargeted metabolomic, and gene expression analyses were performed, as well as an in vivo toxicological study to demonstrate its safety. Probiotic-supplemented rats showed reduced adiposity, an improved inflammatory marker profile, and enhanced glucose tolerance, partly by modulating fecal microbiota composition and regulating the production of novel plasma metabolites, specifically sphingolipid-derived mediators such as ceramides. Moreover, no adverse effects were observed in an in vivo toxicity evaluation of this strain in Wistar rats. Our results unveil the fat-reducing and anti-inflammatory effects of L. sakei CNTA 173 and point out the potential use of this probiotic for the prevention of obesity-related disturbances.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus sakei proBio65	—	Improved Glucose Tolerance	Beneficial	Moderate	View source and enhanced glucose tolerance
Lactobacillus sakei proBio65	—	Improved Inflammatory Biomarkers	Beneficial	Moderate	View source an improved inflammatory marker profile
Lactobacillus sakei proBio65	—	No Observed Toxicity	Beneficial	Small	View source no adverse effects were observed in an in vivo toxicity evaluation of this strain in Wistar rats
Lactobacillus sakei proBio65	—	Reduced Adiposity	Beneficial	Moderate	View source Probiotic-supplemented rats showed reduced adiposity
Lactobacillus sakei proBio65	—	Reduced Ceramide Production	Beneficial	Small	View source regulating the production of novel plasma metabolites, specifically sphingolipid-derived mediators such as ceramides