against pathogenic bacteria".

2023-06-16
Journal of food science and technology 60(10)
- Daraksha Iram
- Manish Singh Sansi
- Sunita Meena
- Anil Kumar Puniya
- Shilpa Vij

PubMed: 37599850
DOI: 10.1007/s13197-023-05776-2

Bioactive peptides (BAPs) have been found to promote health through various mechanisms. Among them, antimicrobial peptides are gaining recognition as promising novel treatments. This study aims to generate BAPs from bovine colostrum whey using the proteolytic activity of Lactobacillus rhamnosus C25 and to evaluate their potential antibacterial efficacy, including their ability to synergistic efficacy against resistant bacteria. Bioactive peptides were successfully generated from lactobacillus culture proteases that were cultivated through batch fermentation. The resulting peptide fractions were then evaluated for their antibacterial efficacy against a selection of strains, including E. coli ATCC25922, S. aureus MTCC1144, Acinetobacter baumannii ATCC 17978, as well as clinically isolated resistant strains of E. coli (ESBL 1384), Acinetobacter 1379, and S. aureus (MRSA 1418). Notably, the peptide fractions with a molecular weight of < 10 kDa (0-10 kDa) significantly increased the membrane permeability of both E. coli (70.30 ± 0.41%) and S. aureus (63.04 ± 0.31%) as assessed by the crystal violet assay. The checkerboard method was utilized to perform synergistic tests with peptides and antibiotics. The peptide fractions with a molecular weight of (< 10 kDa) demonstrated synergistic effects with several antibiotics, including gentamycin, Rifampicin, Levofloxacin, Ciprofloxacin, and Chloramphenicol, against the resistant ESBL 1384 strain, as indicated by ΣFICI values of 0.55, 0.53, 0.52, 0.54, and 0.52, respectively. Furthermore, the HT-29 cell line remained completely unaffected by both peptide fractions. These findings suggest that the < 10 kDa peptide fraction possesses significant antibacterial efficacy against both reference and ESBL 1384 resistant bacterial strain. Additionally, both MRSA 1418 and Acinetobacter 1379 displayed resistance to all fractions tested. To summarize the findings of this study, colostrum whey peptides with a broad spectrum of antimicrobial activity can be efficiently produced through fermentation. This method could prove valuable for both the pharmaceutical and food industries.

Supplementary information

The online version contains supplementary material available at 10.1007/s13197-023-05776-2.

Research Insights

Supplement	Health Outcome	Effect Type	Effect Size
Eastern European Wild Kefir Culture	Enhanced Antibacterial Effect	Beneficial	Moderate
Eastern European Wild Kefir Culture	Enhanced Antimicrobial Activity	Beneficial	Moderate
Eastern European Wild Kefir Culture	Increased Bacterial Membrane Permeability	Beneficial	Moderate
Eastern European Wild Kefir Culture	No Cytotoxicity in HT-29 Cells	Beneficial	Small
Eastern European Wild Kefir Culture	Reduced Bacterial Load	Beneficial	Moderate
Eastern European Wild Kefir Culture	Synergistic Antibiotic Effect	Beneficial	Moderate