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Inhibitory effect of Bifidobacterium bifidum ATCC 29521 on colitis and its mechanism.

  • 2020-05
  • The Journal of Nutritional Biochemistry 79
    • A. U. Din
    • Adil Hassan
    • Yuan Zhu
    • Kun Zhang
    • Yi Wang
    • Tianhan Li
    • Yang Wang
    • Guixue Wang

Abstract

Probiotics are known to be beneficial in preventing different diseases in model animals, including inflammatory bowel disease. However, there are few studies on probiotics related to miRNA regulation and disease status. In this article, the beneficial role and mechanisms of the probiotic strain Bifidobacterium bifidum ATCC 29521 have been studied in ulcerative colitis using dextran sodium sulphate (DSS) model. Male C57JBL/6 mice were randomly divided into three groups (n=7): Normal group, dextran sulphate sodium (DSS) group, and Bifido group gavage with Bifidobacterium bifidum ATCC 29521 (2×108 CFU/day). Our strain restored the DSS-caused damage by regulating the expression of immune markers and tight junction proteins (TJP) in the colon; briefly by up-regulating ROS-scavenging enzymes (SOD1, SOD2, CAT, and GPX2), anti-inflammatory cytokines (IL-10, PPARγ, IL-6), TJP's (ZO-1, MUC-2, Claudin-3, and E Cadherin-1) and downregulating inflammatory genes (TNF-α, IL-1β) in Bifido group mice. Inflammatory markers appeared to be regulated by NF-κB nuclear P65 subunit, and its translocation was inhibited in Bifido group mice colon. In addition, the expression of inflammatory genes and colonic TJP were also associated with the restoration of miRNAs (miR-150, miR-155, miR-223) in B. bifidum ATCC 29521 treated Bifido group. The dysbiosis executed by DSS was restored in the Bifido group, demonstrating that B. bifidum ATCC 29521 possessed a probiotic role in our DSS colitis mouse model. B. bifidum ATCC 29521 exhibited its probiotic role through its anti-inflammatory role by modulating miRNA-associated TJP and NF-κB regulation and by partially restoring dysbiosis.

Keywords: Bifidobacterium bifidum; Colitis; Gut microbiota; Tight junction protein; miRNA.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bifidobacterium bifidumImproved Antioxidant DefenseBeneficial
Moderate
Bifidobacterium bifidumReduced Inflammation LevelsBeneficial
Moderate
Bifidobacterium bifidumRestored Intestinal Barrier FunctionBeneficial
Large
Bifidobacterium bifidumRestored Normal MicrobiotaBeneficial
Moderate
Bifidobacterium bifidum HA-132Improved Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium bifidum HA-132Improved Intestinal Barrier FunctionBeneficial
Moderate
Bifidobacterium bifidum HA-132Reduced Colitis SymptomsBeneficial
Large
Bifidobacterium bifidum HA-132Reduced Inflammatory ResponseBeneficial
Large
Bifidobacterium bifidum MAK53B66BImproved Intestinal Barrier FunctionBeneficial
Moderate
Bifidobacterium bifidum MAK53B66BReduced Inflammation LevelsBeneficial
Moderate
Bifidobacterium bifidum MAK53B66BReduced Ulcerative Colitis SymptomsBeneficial
Large
Bifidobacterium bifidum MAK53B66BRestored Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium bifidum SD-6575Altered miRNA ExpressionBeneficial
Moderate
Bifidobacterium bifidum SD-6575Improved Intestinal Barrier FunctionBeneficial
Moderate
Bifidobacterium bifidum SD-6575Reduced Colitis SymptomsBeneficial
Large
Bifidobacterium bifidum SD-6575Reduced Inflammation LevelsBeneficial
Moderate
Bifidobacterium bifidum SD-6575Restored Microbial BalanceBeneficial
Moderate
Bifidobacterium bifidum SD-6576Improved Intestinal Barrier FunctionBeneficial
Moderate
Bifidobacterium bifidum SD-6576Inhibited NF-κB TranslocationBeneficial
Moderate
Bifidobacterium bifidum SD-6576Reduced Inflammation LevelsBeneficial
Moderate
Bifidobacterium bifidum SD-6576Restored Healthy Gut Microbial BalanceBeneficial
Moderate
Bifidobacterium bifidum UABb-10Enhanced Tight Junction IntegrityBeneficial
Moderate
Bifidobacterium bifidum UABb-10Improved Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium bifidum UABb-10Reduced Colitis InflammationBeneficial
Large
Bifidobacterium bifidum VPro 51Inhibited NF-κB TranslocationBeneficial
Moderate
Bifidobacterium bifidum VPro 51Reduced Colonic InflammationBeneficial
Large
Bifidobacterium bifidum VPro 51Regulated microRNA ExpressionBeneficial
Moderate
Bifidobacterium bifidum VPro 51Restored Healthy Gut Microbial BalanceBeneficial
Moderate

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