Skip to main content
Supplement Research and Comparison WebsiteBest Price GuaranteeAbout Us
Supplement Research and Comparison Website

Lactobacillus casei Exerts Anti-Proliferative Effects Accompanied by Apoptotic Cell Death and Up-Regulation of TRAIL in Colon Carcinoma Cells

  • 2016-02-05
  • PLOS ONE 11(2)
    • A. Tiptiri-Kourpeti
    • K. Spyridopoulou
    • Valentina Santarmaki
    • Georgios Aindelis
    • Evgenia Tompoulidou
    • E. Lamprianidou
    • G. Saxami
    • P. Ypsilantis
    • E. Lampri
    • C. Simopoulos
    • I. Kotsianidis
    • A. Galanis
    • Y. Kourkoutas
    • D. Dimitrellou
    • K. Chlichlia

Abstract

Probiotic microorganisms such as lactic acid bacteria (LAB) exert a number of strain-specific health-promoting activities attributed to their immunomodulatory, anti-inflammatory and anti-carcinogenic properties. Despite recent attention, our understanding of the biological processes involved in the beneficial effects of LAB strains is still limited. To this end, the present study investigated the growth-inhibitory effects of Lactobacillus casei ATCC 393 against experimental colon cancer. Administration of live Lactobacillus casei (as well as bacterial components thereof) on murine (CT26) and human (HT29) colon carcinoma cell lines raised a significant concentration- and time-dependent anti-proliferative effect, determined by cell viability assays. Specifically, a dramatic decrease in viability of colon cancer cells co-incubated with 10(9) CFU/mL L. casei for 24 hours was detected (78% for HT29 and 52% for CT26 cells). In addition, live L. casei induced apoptotic cell death in both cell lines as revealed by annexin V and propidium iodide staining. The significance of the in vitro anti-proliferative effects was further confirmed in an experimental tumor model. Oral daily administration of 10(9) CFU live L. casei for 13 days significantly inhibited in vivo growth of colon carcinoma cells, resulting in approximately 80% reduction in tumor volume of treated mice. Tumor growth inhibition was accompanied by L. casei-driven up-regulation of the TNF-related apoptosis-inducing ligand TRAIL and down-regulation of Survivin. Taken together, these findings provide evidence for beneficial tumor-inhibitory, anti-proliferative and pro-apoptotic effects driven by this probiotic LAB strain.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lacticaseibacillus caseiIncreased Apoptotic Cell DeathBeneficial
Large
Lacticaseibacillus caseiIncreased TRAIL ExpressionBeneficial
Moderate
Lacticaseibacillus caseiReduced Colon Cancer Cell ViabilityBeneficial
Large
Lacticaseibacillus caseiReduced Survivin ExpressionBeneficial
Moderate
Lacticaseibacillus caseiReduced Tumor GrowthBeneficial
Large
Lacticaseibacillus casei R0215Increased TRAIL ExpressionBeneficial
Moderate
Lacticaseibacillus casei R0215Induction of Apoptotic Cell DeathBeneficial
Large
Lacticaseibacillus casei R0215Reduced Cancer Cell ViabilityBeneficial
Large
Lacticaseibacillus casei R0215Reduced Colorectal Cancer Cell ProliferationBeneficial
Large
Lacticaseibacillus casei R0215Reduced Tumor GrowthBeneficial
Large
Lactobacillus caseiIncreased Colorectal Cancer Cell DeathBeneficial
Large
Lactobacillus caseiIncreased TRAIL ExpressionBeneficial
Moderate
Lactobacillus caseiReduced Cancer Cell ViabilityBeneficial
Large
Lactobacillus caseiReduced Colorectal Cancer Cell ProliferationBeneficial
Large
Lactobacillus caseiReduced Tumor GrowthBeneficial
Large
Lactobacillus casei LC03Induction of Apoptotic Cell DeathBeneficial
Large
Lactobacillus casei LC03Inhibition of Colon Carcinoma Cell GrowthBeneficial
Large
Lactobacillus casei LC03Reduced Colon Cancer Cell ProliferationBeneficial
Large
Lactobacillus casei PXN 37Increased Apoptotic Cell DeathBeneficial
Large
Lactobacillus casei PXN 37Increased TRAIL ExpressionBeneficial
Moderate
Lactobacillus casei PXN 37Reduced Colon Carcinoma Cell ViabilityBeneficial
Large
Lactobacillus casei PXN 37Reduced Survivin ExpressionBeneficial
Moderate
Lactobacillus casei PXN 37Reduced Tumor GrowthBeneficial
Large
Lactobacillus casei R0215Increased Apoptosis in Colorectal Cancer CellsBeneficial
Large
Lactobacillus casei R0215Reduced Colon Cancer Cell ProliferationBeneficial
Large
Lactobacillus casei R0215Reduced Tumor VolumeBeneficial
Large
Lactobacillus casei SD-5213Increased TRAIL ExpressionBeneficial
Moderate
Lactobacillus casei SD-5213Induction of Apoptotic Cell DeathBeneficial
Large
Lactobacillus casei SD-5213Reduced Cell ViabilityBeneficial
Large
Lactobacillus casei SD-5213Reduced Tumor GrowthBeneficial
Large
Lactobacillus casei SD-5842Increased TRAIL ExpressionBeneficial
Moderate
Lactobacillus casei SD-5842Induction of Apoptotic Cell DeathBeneficial
Large
Lactobacillus casei SD-5842Reduced Cancer Cell ViabilityBeneficial
Large
Lactobacillus casei SD-5842Reduced Survivin ExpressionBeneficial
Moderate
Lactobacillus casei SD-5842Reduced Tumor VolumeBeneficial
Large
Lactobacillus casei subsp. caseiAnti-Proliferative Effects on Colon CarcinomaBeneficial
Large
Lactobacillus casei subsp. caseiIncreased TRAIL ExpressionBeneficial
Moderate
Lactobacillus casei subsp. caseiInduction of Apoptotic Cell DeathBeneficial
Large
Lactobacillus casei subsp. caseiReduced Survivin ExpressionBeneficial
Moderate
Lactobacillus casei subsp. caseiReduced Tumor VolumeBeneficial
Large
Lactobacillus casei UALc-03Increased TRAIL ExpressionBeneficial
Moderate
Lactobacillus casei UALc-03Induction of Apoptotic Cell DeathBeneficial
Large
Lactobacillus casei UALc-03Inhibition of Colon Carcinoma Cell GrowthBeneficial
Large
Lactobacillus casei UALc-03Reduced Tumor VolumeBeneficial
Large
Lactobacillus casei VK4Induction of Apoptotic Cell DeathBeneficial
Large
Lactobacillus casei VK4Reduced Cancer Cell ViabilityBeneficial
Large
Lactobacillus casei VK4Reduced Tumor GrowthBeneficial
Large
⬆ Back to top
Unsubscribe anytime. See our Privacy Policy.
Pillser
Supplement Research and Comparison Website: evidence-based information about supplements, their benefits, potential risks, and their efficacy.

Join Our Community

Statements on this website have not been reviewed by the U.S. Food and Drug Administration. These products are not meant to diagnose, treat, cure, or prevent any disease. The information here is not a replacement for personal medical advice.