Lactobacillus crispatus represses vaginolysin expression by BV associated Gardnerella vaginalis and reduces cell cytotoxicity.

2018-04
Anaerobe 50
- J. Castro
- A. Martins
- M. E. Rodrigues
- N. Cerca

PubMed: 29427630
DOI: 10.1016/j.anaerobe.2018.01.014

Study Design

Methods: In vitro experiment

Abstract

Using a chemically-defined medium simulating genital tract secretions, we have shown that pre-adhering Lactobacillus crispatus to Hela epithelial cells reduced cytotoxicity caused by Gardnerella vaginalis. This effect was associated to the expression of vaginolysin and was specific to L. crispatus interference, as other vaginal facultative anaerobes had no protective effect.

Keywords: Bacterial vaginosis; G. vaginalis; L. crispatus; Sialidase; Vaginolysin.

Research Insights

Lactobacillus crispatus LV5→Reduced Cytotoxicity
Using a chemically-defined medium simulating genital tract secretions, we have shown that pre-adhering Lactobacillus crispatus to Hela epithelial cells reduced cytotoxicity caused by Gardnerella vaginalis.
Effect
Beneficial
Effect size
Moderate
Lactobacillus crispatus VPro 31→Reduced Cytotoxicity
Using a chemically-defined medium simulating genital tract secretions, we have shown that pre-adhering Lactobacillus crispatus to Hela epithelial cells reduced cytotoxicity caused by Gardnerella vaginalis.
Effect
Beneficial
Effect size
Moderate