Lactobacillus plantarum YS2 (yak yogurt Lactobacillus) exhibited an activity to attenuate activated carbon-induced constipation in male Kunming mice.
- 2019-01
- Journal of Dairy Science 102(1)
- Xin Zhao
- Y. Qian
- Guijie Li
- Ruokun Yi
- Kun-Young Park
- Jia-Le Song
- PubMed: 30527985
- DOI: 10.3168/jds.2018-15206
Abstract
The present study investigated the effects of Lactobacillus plantarum YS2 (LP-YS2) that was isolated from yak yogurt on activated carbon-induced constipation in Kunming (KM) mice. The KM mice were orally administered LP-YS2 and reference strain Lactobacillus delbrueckii ssp. bulgaricus. Administration of LP-YS2 [1.0 × 109 cfu/kg of body weight (BW)] promoted gastrointestinal peristalsis and reduced the first black stool defecation time (129 min), which clearly defines attenuation of the voiding difficulty in mice with constipation. The LP-YS2 treatment also increased the serum level of motilin (MTL; 178.2 pg/mL), gastrin (69.4 pg/mL), acetylcholine (Ach; 30.1 pg/mL), substance P (SP; 57.6 pg/mL), and vasoactive intestinal peptide (VIP; 53.2 pg/mL) and reduced the somatostatin (SS, 32.6 pg/mL) levels compared with the L. delbrueckii ssp. bulgaricus treatment (MTL, 139.7 pg/mL; gastrin, 43.1 pg/mL; Ach, 15.9 pg/mL; SP, 43.6 pg/mL; VIP, 32.3 pg/mL; SS, 55.1 pg/mL) and the control (MTL, 105.3 pg/mL; gastrin, 26.7 pg/mL; Ach, 9.7 pg/mL; SP, 30.2 pg/mL; VIP, 21.0 pg/mL; SS, 70.5 pg/mL). The LP-YS2 treatment significantly increased the colonic mRNA and protein expression of c-Kit (CD117, cluster of differentiation 117; 2.87 times mRNA expression of the control group), stem cell factor (30.40 times mRNA expression of the control group), and glial cell-derived neurotrophic factor (29.97 times mRNA expression of the control group) in mice with constipation. In addition, LP-YS2 reduced the expression of transient receptor potential vanilloid 1 (0.42 times mRNA expression of the control group) and nitric oxide synthase (0.49 times mRNA expression of the control group) in constipated mice. These results demonstrate that LP-YS2 was able to attenuate the activated carbon-induced constipation in KM mice.
Keywords: Lactobacillus fermentum YS2; activated carbon; constipation; gastrointestinal transit.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Improved Gastrointestinal Transit | Beneficial | Large |
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Increased Intestinal GLP-1 Levels | Beneficial | Large |
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Reduced Inflammatory Marker Expression | Beneficial | Large |
| Lactobacillus plantarum LP-01 | Elevated Serum Acetylcholine Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Elevated Serum Substance P Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Improved Gastrointestinal Peristalsis | Beneficial | Large |
| Lactobacillus plantarum LP-01 | Increased Colonic c-Kit Expression | Beneficial | Large |
| Lactobacillus plantarum LP-01 | Increased Expression of Glial Cell-Derived Neurotrophic Factor in the Colon | Beneficial | Large |
| Lactobacillus plantarum LP-01 | Increased Serum Gastrin Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Increased Serum Motilin Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Increased Vasoactive Intestinal Peptide Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced Colonic iNOS Expression | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced Serum Somatostatin Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced TRPV1 Receptor Expression | Beneficial | Moderate |