Lactobacillus rhamnosus GG (ATCC 53103) for the Management of Infantile Colic: A Randomized Controlled Trial
- 2020-06-05
- Nutrients 12(6)
- F. Savino
- P. Montanari
- I. Galliano
- V. Daprá
- M. Bergallo
- PubMed: 32517123
- DOI: 10.3390/nu12061693
Abstract
Background: The aim of this study was to investigate the efficacy of Lactobacillus rhamnosus ATCC 53103 together with the maternal diet avoidance of cow's milk in treating infantile colic.
Methods: Forty-five colicky breastfed infants were consecutively randomized to receive L. rhamnosus for 28 days at a dosage of 5 × 109 cfu per day or placebo. Faecal samples were collected from each subject before starting supplementation and at the end of the study period and were immediately analysed. Faecal calprotectin was detected via a quantitative assay. The total bacterial load and selected bacterial species were evaluated using real-time TaqMan PCR.
Results: After supplementation for 28 days with Lactobacillus rhamnosus ATCC 53103, median full-force daily crying was reduced (104 versus 242 min, p < 0.001) and the values of faecal calprotectin decreased significantly (p = 0.026). Furthermore, the probiotic increased the abundance of Lactobacillus (p = 0.048) and total bacteria (p = 0.040); all these effects were not observed in the placebo group.
Conclusion: Infants treated with Lactobacillus rhamnosus ATCC 53103 for 28 days, in association with the elimination of cow's milk from the maternal diet, presented some interesting features related to the effect of this probiotic treatment: reductions in crying time and faecal calprotectin, with increased total bacteria and Lactobacillus. To validate these results, a double-blind, placebo-controlled trial on a larger cohort is required.
Keywords: Lactobacillus; Lactobacillus rhamnosus (ATCC 53103); faecal calprotectin; infantile colic; total bacteria.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus rhamnosus | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus | Reduced Crying in Infants | Beneficial | Large |
| Lactobacillus rhamnosus | Reduced Fecal Calprotectin Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus CLR1505 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus CLR1505 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus CLR1505 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus CLR1505 | Reduced Fecal Calprotectin Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus CRL 1505 | Increased Lactobacilli Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus CRL 1505 | Increased Total Bacterial Count | Beneficial | Moderate |
| Lactobacillus rhamnosus CRL 1505 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus CRL 1505 | Reduced Fecal Calprotectin Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus DSM 6594 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus DSM 6594 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus DSM 6594 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus DSM 6594 | Reduced Fecal Calprotectin Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus DSM33560 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus DSM33560 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus DSM33560 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus DSM33560 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus GG | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus GG | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus GG | Reduced Crying in Infants | Beneficial | Large |
| Lactobacillus rhamnosus GG | Reduced Fecal Calprotectin Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus GR-1 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus GR-1 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus GR-1 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus GR-1 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-11 | Increased Total Bacterial Abundance | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-111 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-111 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-111 | Reduced Crying in Infants | Beneficial | Large |
| Lactobacillus rhamnosus HA-111 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-114 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-114 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-114 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus HA-114 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-500 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-500 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-500 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus HA-500 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus IMC 501 | Increased Fecal Lactobacillus Abundance | Beneficial | Moderate |
| Lactobacillus rhamnosus IMC 501 | Increased Total Fecal Bacterial Counts | Beneficial | Moderate |
| Lactobacillus rhamnosus IMC 501 | Reduced Crying in Infants | Beneficial | Large |
| Lactobacillus rhamnosus IMC 501 | Reduced Fecal Calprotectin Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus LBV 96 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus LBV 96 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus LBV 96 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus LBV96 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus LBV96 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus LBV96 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus LBV96 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus LCS-742 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus LCS-742 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus LCS-742 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus LCS-742 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus Lr-32 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus Lr-32 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus Lr-32 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus Lr-32 | Reduced Fecal Calprotectin Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus LR04 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus LR04 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus LR04 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus LR04 | Reduced Fecal Calprotectin Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus LR05 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus LR05 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus LR05 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus LR05 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus LR06 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus LRa05 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus MAK79L08R | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus MAK79L08R | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus MAK79L08R | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus MAK79L08R | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus NH001 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus NH001 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus NH001 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus NH001 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus PXN 54 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus PXN 54 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus PXN 54 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus PXN 54 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus R0011 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus R0011 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus R0011 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus R0011 | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus R0343ND | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus R0343ND | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus R0343ND | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus R0343ND | Reduced Faecal Calprotectin | Beneficial | Moderate |
| Lactobacillus rhamnosus SD-5217 | Increased Total Bacterial Count | Beneficial | Moderate |
| Lactobacillus rhamnosus SD-5217 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus SD-5217 | Reduced Fecal Calprotectin Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus UALr-06 | Increased Lactobacillus Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus UALr-18 | Increased Fecal Lactobacilli | Beneficial | Moderate |
| Lactobacillus rhamnosus UALr-18 | Increased Total Bacterial Load | Beneficial | Moderate |
| Lactobacillus rhamnosus UALr-18 | Reduced Crying Duration | Beneficial | Large |
| Lactobacillus rhamnosus UALr-18 | Reduced Faecal Calprotectin | Beneficial | Moderate |