Skip to main content

Lactobacillus rhamnosus GG supernatant promotes intestinal mucin production through regulating 5-HT4R and gut microbiota.

  • 2022
  • Food & Function 13(23)
    • Y. Gu
    • Xiali Qin
    • Guoqiong Zhou
    • Chen Wang
    • Chenlu Mu
    • Xiang Liu
    • Weilong Zhong
    • Xin Xu
    • Bangmao Wang
    • Kui Jiang
    • Jinghua Liu
    • H. Cao

Abstract

Lactobacillus rhamnosus GG (LGG) is a well-known probiotic widely used in foods and drugs. It has been reported that LGG can improve bowel dysfunction in gastrointestinal motility disorders, such as constipation; however, the specific mechanisms remain unclear. The colonic mucus layer is mainly composed of mucin secreted by goblet cells, which plays important roles in lubricating colonic contents and maintaining normal defecation function. It has been reported that increased mucin production is beneficial for relieving constipation symptoms. In this study, we aimed to investigate the role of LGG in regulating intestinal mucin production and the associated mechanisms. Six-week-old C57BL/6J mice were randomized into 3 groups, and were treated with De-Man Rogosa and Sharpe broth (MRS group), tegaserod maleate (tegaserod group) and LGG supernatant (LGGs group) by gavage, respectively. After treatments, defecation parameters, intestinal mucin-2 (MUC2) and serotonin 4 receptor (5-HT4R), goblet cells, and microbiota composition of the mice in each group were assessed. In comparison with the MRS group, higher fecal water content and increased fecal pellet number were found in the tegaserod group and LGGs group. Moreover, LGGs increased the number of goblet cells and upregulated the expression of 5-HT4R and MUC2 in the mouse colon. In addition, Alcian Blue Periodic acid Schiff staining showed that activated 5-HT4R enhanced intestinal MUC2 secretion. Further exploration of the mechanism discovered that LGGs upregulated intestinal S100A10, which was found to be involved in regulating 5-HT4R expression. Furthermore, gut microbiota analysis showed the higher abundance of Alistipes, Allobaculum, Desulfovibrio, and Clostridium XlVa in the LGGs group, which have been reported to be involved in regulating gut motility and the intestinal barrier, and alleviating bowel dysfunction. Interestingly, gut dysbiosis was present in the tegaserod group. It is noteworthy that the fecal microbiota transplanted from LGGs-treated mice significantly improved the gut dysmotility in a constipation mouse model. Our results suggested that LGGs could upregulate 5-HT4R to promote MUC2 production, as well as modulate the gut microbiota, thus improving the defecation function in mice. This finding might provide evidence for the application of diet supplementary LGG in relieving gastrointestinal motility disorders.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus rhamnosus GGImproved Bowel FunctionBeneficial
Moderate
Lactobacillus rhamnosus GGImproved Gut Microbiota CompositionBeneficial
Moderate
Lactobacillus rhamnosus GGImproved Gut MotilityBeneficial
Large
Lactobacillus rhamnosus GGIncreased Mucin ProductionBeneficial
Large
Lactobacillus rhamnosus LR04Enhanced Intestinal MUC2 SecretionBeneficial
Moderate
Lactobacillus rhamnosus LR04Improved Defecation ConditionBeneficial
Large
Lactobacillus rhamnosus LR04Improved Gut Microbiome CompositionBeneficial
Moderate
Lactobacillus rhamnosus LR04Increased Mucin ProductionBeneficial
Large
Lactobacillus rhamnosus LR05Altered Gut MicrobiotaBeneficial
Moderate
Lactobacillus rhamnosus LR05Improved Defecation ConditionBeneficial
Large
Lactobacillus rhamnosus LR05Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus rhamnosus LR05Increased Mucin ProductionBeneficial
Large
Lactobacillus rhamnosus LR06Enhanced Intestinal MUC2 SecretionBeneficial
Moderate
Lactobacillus rhamnosus LR06Improved Intestinal MotilityBeneficial
Moderate
Lactobacillus rhamnosus LR06Increased 5-HT4R ExpressionBeneficial
Moderate
Lactobacillus rhamnosus LR06Increased Beneficial Gut BacteriaBeneficial
Large
Lactobacillus rhamnosus LR06Increased Fecal Pellet CountBeneficial
Moderate
Lactobacillus rhamnosus LR06Increased Fecal Water ContentBeneficial
Moderate
Lactobacillus rhamnosus LR06Increased Goblet Cell CountBeneficial
Moderate
Lactobacillus rhamnosus LR06Increased MUC-2 ExpressionBeneficial
Moderate
Lactobacillus rhamnosus LR06Increased S100A10 ExpressionBeneficial
Moderate
Lactobacillus rhamnosus Lr-32Improved Defecation ConditionBeneficial
Large
Lactobacillus rhamnosus Lr-32Improved Gut Microbiome CompositionBeneficial
Moderate
Lactobacillus rhamnosus Lr-32Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus rhamnosus Lr-32Increased Goblet Cell CountBeneficial
Moderate
Lactobacillus rhamnosus Lr-32Increased Mucin ProductionBeneficial
Large
Lactobacillus rhamnosus lysate powderEnhanced Intestinal Mucin ProductionBeneficial
Large
Lactobacillus rhamnosus lysate powderImproved Bowel FunctionBeneficial
Moderate
Lactobacillus rhamnosus lysate powderImproved Gut MotilityBeneficial
Large
⬆ Back to top
Pillser
Supplement Research and Comparison Website: evidence-based information about supplements, their benefits, potential risks, and their efficacy.
Receive updates about our products, services, sales, and special offers. Unsubscribe anytime. See our Privacy Policy for details on how we handle your information.

Join Our Community

Use support@pillser.com to get in touch.