Oral administration of heat-killed Lactobacillus brevis SBC8803 elevates the ratio of acyl/des-acyl ghrelin in blood and increases short-term food intake.
- 2019-07-10
- Beneficial microbes 10(6)
- PubMed: 31190555
- DOI: 10.3920/bm2018.0091
Study Design
- Population
- primary mouse stomach cells; rats
- Methods
- Treating cultured cells and experimental animals with heat-killed Lactobacillus brevis SBC8803 (hk-SBC8803) and assessing ghrelin secretion, expression of ghrelin-related genes, and food intake; oral administration of 500 mg/kg hk-SBC8803 to rats
- Rigorous Journal
- Animal Study
It is known that gastrointestinal microbiota, probiotics and heat-killed microbes can regulate intestinal immunity; however, their effect on the secretion of gastrointestinal hormones is unclear. The secretion of gastrointestinal hormones can be mediated by the elevation of intracellular Ca2+ concentration, suggesting that these hormones may act through common mechanisms. We have previously shown that heat-killed Lactobacillus brevis SBC8803 (hk-SBC8803) induced the secretion of serotonin and elevated intracellular Ca2+ concentration in serotonin-producing RIN-14B cells, suggesting that hk-SBC8803 could potentially cause the same effect on other gastrointestinal hormones, including hunger hormone ghrelin. Here, we tested this hypothesis by treating cultured cells and experimental animals with hk-SBC8803 and assessing ghrelin secretion, expression of ghrelin-related genes, and food intake. The results indicated that hk-SBC8803 treatment for 30 min significantly upregulated the secretion of acyl ghrelin (active form) (P=0.046) and mRNA expression of the Syt3 (synaptotagmin 3) gene related to ghrelin exocytosis (P<0.05) in primary mouse stomach cells. In addition, oral administration of 500 mg/kg hk-SBC8803 to rats tended to upregulate acyl ghrelin concentration (P=0.10) and significantly increased the ratio of acyl to des-acyl (inactive) ghrelin (P=0.027) in blood, which corresponded to a tendency of stimulating food intake (P=0.087) at 30 min post-treatment. However, when in order to minimise individual differences we normalised the data on food intake to those on one-day food intake prior to food deprivation, the resultant food intake ratio showed a significant increase (by 5% compared to control; P=0.032) at 30 min after hk-SBC8803 treatment, indicating that hk-SBC8803 administration stimulated rats to take more food during the first meal after fasting. These results suggest that hk-SBC8803 induces short-term ghrelin secretion and transiently increases appetite, which is an important effect for individuals with low energy intake.
Research Insights
| Supplement | Dose | Health Outcome | Effect Type | Effect Size | Source |
|---|---|---|---|---|---|
| Lactobacillus brevis SBC8803 | — | Increased Acyl Ghrelin Secretion | Beneficial | Small | View sourcehk-SBC8803 treatment for 30 min significantly upregulated the secretion of acyl ghrelin (active form) ... in primary mouse stomach cells |
| Lactobacillus brevis SBC8803 | — | Increased Acyl/Des-Acyl Ghrelin Ratio | Beneficial | Small | View sourcesignificantly increased the ratio of acyl to des-acyl (inactive) ghrelin in blood |
| Lactobacillus brevis SBC8803 | — | Increased Appetite | Beneficial | Small | View sourceoral administration of 500 mg/kg hk-SBC8803 to rats tended to upregulate acyl ghrelin concentration ... and significantly increased the ratio of acyl to des-acyl (inactive) ghrelin ... which corresponded to a tendency of stimulating food intake ... at 30 min post-treatment |