Oral administration of Lactobacillus fermentum I5007 favors intestinal development and alters the intestinal microbiota in formula-fed piglets.

2014-01-15
Journal of Agricultural and Food Chemistry 62(4)
- Hong Liu
- Jiang Zhang
- Shihai Zhang
- Fengjuan Yang
- P. Thacker
- Guolong Zhang
- S. Qiao
- Xi Ma

PubMed: 24404892
DOI: 10.1021/jf403288r

Study Design

Type: Randomized Controlled Trial (RCT)
Sample size: n = 2
Population: Neonatal piglets
Methods: Controlled experimental study

Highly Cited
Animal Study

Abstract

The present study was conducted to evaluate the effects of early administration of Lactobacillus fermentum I5007 on intestinal development and microbial composition in the gastrointestinal tract using a neonatal piglet model. Full-term 4 day old piglets, fed with milk replacer, were divided into a control group (given placebo of 0.1% peptone water) and a L. fermentum I5007 group (dosed daily with 6 × 10(9) CFU/mL L. fermentum I5007). The experiment lasted 14 days. On day 14, a significant increase in the jejunum villous height (583 ± 33 vs 526 ± 18) and increases in the concentrations of butyrate (7.55 ± 0.55 vs 5.33 ± 0.39) and branched chain fatty acids in the colonic digesta were observed in piglets in the L. fermentum I5007 treatment (P < 0.05). mRNA expression of IL-1β (1.29 ± 0.29 vs. 0.62 ± 0.07) in the ileum were lower after 14 days of treatment with L. fermentum I5007. Denaturing gradient gel electrophoresis (DGGE) revealed that L. fermentum I5007 affected the colonic microbial communities on day 14 and, in particular, reduced numbers of Clostridium sp. L. fermentum I5007 play a positive role in gut development in neonatal piglets by modulating microbial composition, intestinal development, and immune status. L. fermentum I5007 may be useful as a probiotic for application in neonatal piglets.

Research Insights

Lactobacillus fermentum LF61→Increased Butyrate Concentrations in Colonic Digesta
Increases in the concentrations of butyrate (7.55 ± 0.55 vs 5.33 ± 0.39) and branched chain fatty acids in the colonic digesta were observed in piglets in the L. fermentum I5007 treatment (P < 0.05).
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum LF61→Increased Villus Height
On day 14, a significant increase in the jejunum villous height (583 ± 33 vs 526 ± 18) ... were observed in piglets in the L. fermentum I5007 treatment (P < 0.05).
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum LF61→Reduced Clostridium Counts
Denaturing gradient gel electrophoresis (DGGE) revealed that L. fermentum I5007 affected the colonic microbial communities on day 14 and, in particular, reduced numbers of Clostridium sp.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum MAK20L13F→Increased Branched Chain Fatty Acids in Colonic Digesta
On day 14, ... increases in the concentrations of ... branched chain fatty acids in the colonic digesta were observed in piglets in the L. fermentum I5007 treatment (P < 0.05).
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum MAK20L13F→Increased Fecal Butyric Acid Concentration
On day 14, ... increases in the concentrations of butyrate (7.55 ± 0.55 vs 5.33 ± 0.39) ... were observed in piglets in the L. fermentum I5007 treatment (P < 0.05).
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum MAK20L13F→Increased Villus Height
On day 14, a significant increase in the jejunum villous height (583 ± 33 vs 526 ± 18) ... were observed in piglets in the L. fermentum I5007 treatment (P < 0.05).
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum MAK20L13F→Reduced Colonic Clostridium Species
Denaturing gradient gel electrophoresis (DGGE) revealed that L. fermentum I5007 affected the colonic microbial communities on day 14 and, in particular, reduced numbers of Clostridium sp.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum UALf-32→Enhanced Intestinal Development
On day 14, a significant increase in the jejunum villous height (583 ± 33 vs 526 ± 18) was observed in piglets in the L. fermentum I5007 treatment (P < 0.05).
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum UALf-32→Increased Butyrate Levels
On day 14, increases in the concentrations of butyrate (7.55 ± 0.55 vs 5.33 ± 0.39) in the colonic digesta were observed in piglets in the L. fermentum I5007 treatment (P < 0.05).
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum UALf-32→Reduced Inflammatory Response
mRNA expression of IL-1β (1.29 ± 0.29 vs. 0.62 ± 0.07) in the ileum were lower after 14 days of treatment with L. fermentum I5007.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum VPro 14→Altered Colonic Microbial Communities
Denaturing gradient gel electrophoresis (DGGE) revealed that L. fermentum I5007 affected the colonic microbial communities on day 14 and, in particular, reduced numbers of Clostridium sp.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum VPro 14→Increased Branched Chain Fatty Acids in Colonic Digesta
On day 14, ... increases in the concentrations of ... branched chain fatty acids in the colonic digesta were observed in piglets in the L. fermentum I5007 treatment (P < 0.05).
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum VPro 14→Increased Fecal Butyric Acid Concentration
On day 14, ... increases in the concentrations of butyrate (7.55 ± 0.55 vs 5.33 ± 0.39) ... were observed in piglets in the L. fermentum I5007 treatment (P < 0.05).
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum VPro 14→Increased Villus Height
On day 14, a significant increase in the jejunum villous height (583 ± 33 vs 526 ± 18) ... were observed in piglets in the L. fermentum I5007 treatment (P < 0.05).
Effect
Beneficial
Effect size
Moderate