Oral administration of Lactobacillus fermentum I5007 favors intestinal development and alters the intestinal microbiota in formula-fed piglets.
- 2014-01-15
- Journal of Agricultural and Food Chemistry 62(4)
- Hong Liu
- Jiang Zhang
- Shihai Zhang
- Fengjuan Yang
- P. Thacker
- Guolong Zhang
- S. Qiao
- Xi Ma
- PubMed: 24404892
- DOI: 10.1021/jf403288r
Abstract
The present study was conducted to evaluate the effects of early administration of Lactobacillus fermentum I5007 on intestinal development and microbial composition in the gastrointestinal tract using a neonatal piglet model. Full-term 4 day old piglets, fed with milk replacer, were divided into a control group (given placebo of 0.1% peptone water) and a L. fermentum I5007 group (dosed daily with 6 × 10(9) CFU/mL L. fermentum I5007). The experiment lasted 14 days. On day 14, a significant increase in the jejunum villous height (583 ± 33 vs 526 ± 18) and increases in the concentrations of butyrate (7.55 ± 0.55 vs 5.33 ± 0.39) and branched chain fatty acids in the colonic digesta were observed in piglets in the L. fermentum I5007 treatment (P < 0.05). mRNA expression of IL-1β (1.29 ± 0.29 vs. 0.62 ± 0.07) in the ileum were lower after 14 days of treatment with L. fermentum I5007. Denaturing gradient gel electrophoresis (DGGE) revealed that L. fermentum I5007 affected the colonic microbial communities on day 14 and, in particular, reduced numbers of Clostridium sp. L. fermentum I5007 play a positive role in gut development in neonatal piglets by modulating microbial composition, intestinal development, and immune status. L. fermentum I5007 may be useful as a probiotic for application in neonatal piglets.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus fermentum | Altered Gut Microbiota Composition | Beneficial | Large |
| Lactobacillus fermentum | Improved Intestinal Development | Beneficial | Moderate |
| Lactobacillus fermentum | Increased Butyrate Levels | Beneficial | Large |
| Lactobacillus fermentum | Reduced Inflammatory Marker IL-1β | Beneficial | Moderate |
| Lactobacillus fermentum CECT5716 | Altered Microbial Composition | Beneficial | Moderate |
| Lactobacillus fermentum CECT5716 | Improved Intestinal Development | Beneficial | Moderate |
| Lactobacillus fermentum CECT5716 | Increased Butyrate Levels | Beneficial | Large |
| Lactobacillus fermentum CECT5716 | Reduced Inflammation Levels | Beneficial | Moderate |
| Lactobacillus fermentum HA-179 | Improved Gut Microbiota Composition | Beneficial | Moderate |
| Lactobacillus fermentum HA-179 | Improved Intestinal Development | Beneficial | Moderate |
| Lactobacillus fermentum HA-179 | Increased Branched Chain Fatty Acid Concentration | Beneficial | Small |
| Lactobacillus fermentum HA-179 | Increased Butyrate Levels | Beneficial | Large |
| Lactobacillus fermentum HA-179 | Reduced Inflammatory Marker IL-1β Expression | Beneficial | Moderate |
| Lactobacillus fermentum Lf-33 | Altered Microbial Composition | Beneficial | Moderate |
| Lactobacillus fermentum Lf-33 | Enhanced Immune Status | Beneficial | Moderate |
| Lactobacillus fermentum Lf-33 | Improved Intestinal Development | Beneficial | Moderate |
| Lactobacillus fermentum Lf-33 | Increased Branched-Chain Fatty Acids Concentration | Beneficial | Moderate |
| Lactobacillus fermentum Lf-33 | Increased Butyrate Levels | Beneficial | Large |
| Lactobacillus fermentum LF61 | Increased Butyrate Concentrations in Colonic Digesta | Beneficial | Moderate |
| Lactobacillus fermentum LF61 | Increased Villus Height | Beneficial | Moderate |
| Lactobacillus fermentum LF61 | Reduced Clostridium Counts | Beneficial | Moderate |
| Lactobacillus fermentum LF61 | Reduced IL-1β mRNA Expression in the Ileum | Beneficial | Moderate |
| Lactobacillus fermentum MAK20L13F | Increased Branched Chain Fatty Acids in Colonic Digesta | Beneficial | Moderate |
| Lactobacillus fermentum MAK20L13F | Increased Fecal Butyric Acid Concentration | Beneficial | Moderate |
| Lactobacillus fermentum MAK20L13F | Increased Villus Height | Beneficial | Moderate |
| Lactobacillus fermentum MAK20L13F | Reduced Colonic Clostridium Species | Beneficial | Moderate |
| Lactobacillus fermentum MAK20L13F | Reduced mRNA Expression of IL-1β in Ileum | Beneficial | Moderate |
| Lactobacillus fermentum ME-3 | Altered Gut Microbiota | Beneficial | Moderate |
| Lactobacillus fermentum ME-3 | Improved Immunity Status | Beneficial | Moderate |
| Lactobacillus fermentum ME-3 | Improved Intestinal Development | Beneficial | Moderate |
| Lactobacillus fermentum ME-3 | Increased Butyrate Levels | Beneficial | Large |
| Lactobacillus fermentum UALf-32 | Altered Intestinal Microbiota Composition | Beneficial | Moderate |
| Lactobacillus fermentum UALf-32 | Enhanced Intestinal Development | Beneficial | Moderate |
| Lactobacillus fermentum UALf-32 | Increased Butyrate Levels | Beneficial | Large |
| Lactobacillus fermentum UALf-32 | Reduced Inflammatory Response | Beneficial | Moderate |
| Lactobacillus fermentum VPro 14 | Altered Colonic Microbial Communities | Beneficial | Moderate |
| Lactobacillus fermentum VPro 14 | Increased Branched Chain Fatty Acids in Colonic Digesta | Beneficial | Moderate |
| Lactobacillus fermentum VPro 14 | Increased Fecal Butyric Acid Concentration | Beneficial | Moderate |
| Lactobacillus fermentum VPro 14 | Increased Villus Height | Beneficial | Moderate |
| Lactobacillus fermentum VPro 14 | Reduced mRNA Expression of IL-1β in Ileum | Beneficial | Moderate |