Oral administration of Lactobacillus rhamnosus GG to newborn piglets augments gut barrier function in pre-weaning piglets

2019-02
Journal of Zhejiang University-SCIENCE B 20(2)
- Yang Wang
- L. Gong
- Yan-ping Wu
- Z. Cui
- Yong-qiang Wang
- Yi Huang
- Xiao-ping Zhang
- Wei-fen Li

PubMed: 30666850
DOI: 10.1631/jzus.B1800022

Study Design

Type: Randomized Controlled Trial (RCT)
Sample size: n = 24
Population: Newborn piglets
Methods: Controlled experimental study

Animal Study

Abstract

To understand the effects of Lactobacillus rhamnosus GG (ATCC 53103) on intestinal barrier function in pre-weaning piglets under normal conditions, twenty-four newborn littermate piglets were randomly divided into two groups. Piglets in the control group were orally administered with 2 mL 0.1 g/mL sterilized skim milk while the treatment group was administered the same volume of sterilized skim milk with the addition of viable L. rhamnosus at the 1st, 3rd, and 5th days after birth. The feeding trial was conducted for 25 d. Results showed that piglets in the L. rhamnosus group exhibited increased weaning weight and average daily weight gain, whereas diarrhea incidence was decreased. The bacterial abundance and composition of cecal contents, especially Firmicutes, Bacteroidetes, and Fusobacteria, were altered by probiotic treatment. In addition, L. rhamnosus increased the jejunal permeability and promoted the immunologic barrier through regulating antimicrobial peptides, cytokines, and chemokines via Toll-like receptors. Our findings indicate that oral administration of L. rhamnosus GG to newborn piglets is beneficial for intestinal health of pre-weaning piglets by improving the biological, physical, and immunologic barriers of intestinal mucosa.

Keywords: Lactobacillus rhamnosus; Gut microbiota; Intestinal physical barrier; Intestinal immunological barrier; Piglet.

Research Insights

Lactobacillus rhamnosus LCS-742→Improved Immunologic Barrier Function
...promoted the immunologic barrier through regulating antimicrobial peptides, cytokines, and chemokines via Toll-like receptors.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus LCS-742→Increased Jejunal Permeability
In addition, L. rhamnosus increased the jejunal permeability...
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus LCS-742→Increased Weaning Weight
Results showed that piglets in the L. rhamnosus group exhibited increased weaning weight and average daily weight gain...
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus LCS-742→Reduced Diarrhea Rate
...whereas diarrhea incidence was decreased.
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus MAK79L08R→Improved Gut Permeability
In addition, L. rhamnosus increased the jejunal permeability...
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus MAK79L08R→Improved Immunologic Barrier Function
...promoted the immunologic barrier through regulating antimicrobial peptides, cytokines, and chemokines via Toll-like receptors.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus MAK79L08R→Increased Weaning Weight
Results showed that piglets in the L. rhamnosus group exhibited increased weaning weight and average daily weight gain...
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus MAK79L08R→Reduced Diarrhea Rate
...whereas diarrhea incidence was decreased.
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus R0343ND→Improved Average Daily Gain
...and average daily weight gain...
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus R0343ND→Improved Immunologic Barrier Function
...promoted the immunologic barrier through regulating antimicrobial peptides, cytokines, and chemokines via Toll-like receptors.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus R0343ND→Increased Jejunal Permeability
In addition, L. rhamnosus increased the jejunal permeability...
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus R0343ND→Increased Weaning Weight
Results showed that piglets in the L. rhamnosus group exhibited increased weaning weight and average daily weight gain...
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus R0343ND→Reduced Diarrhea Rate
...whereas diarrhea incidence was decreased.
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus UALrh-18→Improved Intestinal Mucosal Immunity
Our findings indicate that oral administration of L. rhamnosus GG to newborn piglets is beneficial for intestinal health of pre-weaning piglets by improving the biological, physical, and immunologic barriers of intestinal mucosa.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus UALrh-18→Reduced Diarrhea Incidence
Results showed that piglets in the L. rhamnosus group exhibited... diarrhea incidence was decreased.
Effect
Beneficial
Effect size
Moderate