- 2021-02-05
- European journal of nutrition 60(6)
- Audrey M Neyrinck
- Julie Rodriguez
- Zhengxiao Zhang
- Benjamin Seethaler
- Cándido Robles Sánchez
- Martin Roumain
- Sophie Hiel
- Laure B Bindels
- Patrice D Cani
- Nicolas Paquot
- Miriam Cnop
- Julie-Anne Nazare
- Martine Laville
- Giulio G Muccioli
- Stephan C Bischoff
- Jens Walter
- Jean-Paul Thissen
- Nathalie M Delzenne
Study Design
- Type
- Randomized Controlled Trial (RCT)
- Sample size
- n = 12
- Population
- obese patients
- Methods
- randomized, placebo-controlled trial; participants received either 16 g/d native inulin (prebiotic n=12) versus maltodextrin (placebo n=12), coupled to dietary advice to consume inulin-rich versus inulin-poor vegetables for 3 months, in addition to dietary caloric restriction
- Blinding
- Double-blind
- Duration
- 3 months
- Funding
- Unclear
Purpose
Inulin-type fructans (ITF) are prebiotic dietary fibre (DF) that may confer beneficial health effects, by interacting with the gut microbiota. We have tested the hypothesis that a dietary intervention promoting inulin intake versus placebo influences fecal microbial-derived metabolites and markers related to gut integrity and inflammation in obese patients.Methods
Microbiota (16S rRNA sequencing), long- and short-chain fatty acids (LCFA, SCFA), bile acids, zonulin, and calprotectin were analyzed in fecal samples obtained from obese patients included in a randomized, placebo-controlled trial. Participants received either 16 g/d native inulin (prebiotic n = 12) versus maltodextrin (placebo n = 12), coupled to dietary advice to consume inulin-rich versus inulin-poor vegetables for 3 months, in addition to dietary caloric restriction.Results
Both placebo and prebiotic interventions lowered energy and protein intake. A substantial increase in Bifidobacterium was detected after ITF treatment (q = 0.049) supporting our recent data obtained in a larger cohort. Interestingly, fecal calprotectin, a marker of gut inflammation, was reduced upon ITF treatment. Both prebiotic and placebo interventions increased the ratio of tauro-conjugated/free bile acids in feces. Prebiotic treatment did not significantly modify fecal SCFA content but it increased fecal rumenic acid, a conjugated linoleic acid (cis-9, trans-11 CLA) with immunomodulatory properties, that correlated notably to the expansion of Bifidobacterium (p = 0.031; r = 0.052).Conclusions
Our study demonstrates that ITF-prebiotic intake during 3 months decreases a fecal marker of intestinal inflammation in obese patients. Our data point to a potential contribution of microbial lipid-derived metabolites in gastro-intestinal dysfunction related to obesity. CLINICALTRIALS.Gov identifier
NCT03852069 (February 22, 2019 retrospectively, registered).