Probiotic administration of lactobacillus rhamnosus GR-1 attenuates atherosclerotic plaque formation in ApoE-/- mice fed with a high-fat diet.
- 2019-04-01
- European review for medical and pharmacological sciences 23(8)
- PubMed: 31081110
- DOI: 10.26355/eurrev_201904_17722
Study Design
- Population
- Eight-week-old ApoE-/- mice fed with a high-fat diet
- Methods
- Eight-week-old ApoE-/- mice were treated with Lactobacillus rhamnosus GR-1 daily for 12 weeks. ApoE-/- mice in the vehicle group and wild type (WT) mice were treated with normal saline. Serum lipid levels, histopathological analysis of the aorta, oxidative and inflammatory indexes and activation of the nuclear factor-kappa B (NF-κB) signaling pathway were examined.
Objective
To investigate the effect of Lactobacillus rhamnosus GR-1 on atherosclerotic progression in apolipoprotein-E knockout (ApoE-/-) mice fed with a high-fat diet and the underlying mechanisms of its action.Materials and methods
Eight-week-old ApoE-/- mice were treated with Lactobacillus rhamnosus GR-1 daily for 12 weeks. ApoE-/- mice in the vehicle group and wild type (WT) mice were treated with normal saline. Serum lipid levels, histopathological analysis of the aorta, oxidative and inflammatory indexes and activation of the nuclear factor-kappa B (NF-κB) signaling pathway were examined.Results
Compared to ApoE-/- mice in the vehicle group, no changes in body weight or serum lipid levels were found in ApoE-/- mice treated with Lactobacillus rhamnosus GR-1. However, the administration of GR-1 slowed down the development of atherosclerosis and reduced plaque formation. Additionally, GR-1 attenuated the development of oxidative stress and chronic inflammation in a dose-dependent manner in ApoE-/- mice fed a high-fat diet. Furthermore, in ApoE-/- mice treated with GR-1, GR-1 was demonstrated to have a role in inhibiting the translocation of NF-κB p65 from the cytoplasm to the nucleus and suppressing the degradation of IκB-α.Conclusions
We showed that the administration of GR-1 decreased atherosclerotic lesion size in ApoE-/- mice by reducing oxidative stress and inflammation. Additionally, the NF-κB signaling pathway might mediate these effects.Research Insights
| Supplement | Dose | Health Outcome | Effect Type | Effect Size | Source |
|---|---|---|---|---|---|
| Lactobacillus rhamnosus GR-1 | — | Reduced Atherosclerotic Plaque Formation | Beneficial | Moderate | View sourcethe administration of GR-1 slowed down the development of atherosclerosis and reduced plaque formation |
| Lactobacillus rhamnosus GR-1 | — | Reduced Inflammation | Beneficial | Moderate | View sourceGR-1 attenuated the development of oxidative stress and chronic inflammation in a dose-dependent manner |
| Lactobacillus rhamnosus GR-1 | — | Reduced Oxidative Stress | Beneficial | Moderate | View sourceGR-1 attenuated the development of oxidative stress and chronic inflammation in a dose-dependent manner |