Skip to main content
Evidence-Based Supplement Research
Evidence-Based Supplement Research
Pillser
Search
Sign UpLog In

Probiotic supplementation attenuates dental pain and inhibits pain-induced cognitive impairment in male rats.

  • 2025-11
  • Archives of oral biology 179

Study Design

Type
Randomized Controlled Trial (RCT)
Sample size
n = 8
Population
Forty-eight male Wistar rats
Methods
Forty-eight male Wistar rats were randomly assigned to six experimental groups (n = 8). The probiotics (10^9 CFU) were orally administered for 14 consecutive days. Capsaicin (100 µg) was used to induce inflammatory pulp nociception. The Morris water maze task was used to evaluate learning and memory performance. Levels of the interleukin-1 beta (IL-1β) and tumour necrosis factor-alpha (TNF-α) cytokines in the animals' trigeminal ganglion (TG) and the brain-derived neurotrophic factor (BDNF), neuropeptide Y (NPY), tropomyosin receptor kinase B (Trk-B) and Cyclooxygenase-2 (COX-2) genes in the animals' hippocampus were determined using western blotting and RT-PCR, respectively.
  • Animal Study

Objectives

The gut-brain axis has emerged as a promising avenue for understanding the bidirectional communication between the gut microbiota and the central nervous system. This study investigated the potential impact of probiotics, Lactobacillus acidophilus (LA-5), Lactobacillus paracasei (L. casei 431), and Bifidobacterium lactis (BB-12), as well as their combination, on dental pulp pain management and cognitive functions.

Design

Forty-eight male Wistar rats were randomly assigned to six experimental groups (n = 8). The probiotics (109 CFU) were orally administered for 14 consecutive days. Capsaicin (100 µg) was used to induce inflammatory pulp nociception. The Morris water maze task was used to evaluate learning and memory performance. Levels of the interleukin-1 beta (IL-1β) and tumour necrosis factor-alpha (TNF-α) cytokines in the animals' trigeminal ganglion (TG) and the brain-derived neurotrophic factor (BDNF), neuropeptide Y (NPY), tropomyosin receptor kinase B (Trk-B) and Cyclooxygenase-2 (COX-2) genes in the animals' hippocampus were determined using western blotting and RT-PCR, respectively.

Results

Intradental application of capsaicin induced nociceptive behavior and increased IL-1β and TNF-α in the TG of rats. Probiotics could attenuate nociception and prevent IL-1β and TNF-α overexpression. Furthermore, pain induced cognitive impairments, decreased BDNF, NPY, and Trk-B and increased COX-2 gene expression in rat hippocampus, which were inhibited by probiotics supplementation.

Conclusions

Our findings suggest that probiotics may play a role in orofacial pain relief and cognitive enhancement in painful situations by modulating gut microbiota composition and influencing protein levels and gene expression in brain regions associated with pain and cognition.

Research Insights

Back to top