Production of lactate and acetate by Lactobacillus coryniformis subsp. torquens DSM 20004(T) in comparison with Lactobacillus amylovorus DSM 20531(T).
- 2015-05
- Journal of biotechnology 202
- Anita Slavica
- Antonija Trontel
- Nuša Jelovac
- Željka Kosovec
- Božidar Šantek
- Srđan Novak
- PubMed: 25617683
- DOI: 10.1016/j.jbiotec.2015.01.014
Lactobacillus coryniformis subsp. torquens DSM20004(T) is a d-lactate producer, with a portion of the d-lactate higher than 99.9% of total lactic acid produced. Acetate was identified as the second end-product that appeared at the end of the exponential growth phase in MRS medium when glucose concentration dropped to 38.41mM (6.92g/L). The acetate production was prolonged to the stationary phase, while the concentration of d-lactate remained constant. Other end-products were not identified by HPLC method. The known metabolic pathways of glucose fermentation in lactic acid bacteria do not produce the particular combination of these two end-products, but besides lactate and acetate also formate, ethanol and CO2 are produced. For comparison, the production of lactate and acetate by a d-/l-lactate producer Lactobacillus amylovorus DSM 20531(T) was also investigated. This strain produced equimolar quantities of d- and l-lactate in the MRS medium. Acetate was produced only when initial concentration of glucose was 55.51mM (10g/L) and production started in the exponential phase when concentration of glucose dropped to 35.52mM (6.40g/L). Similar behavior was observed with the initial concentration of maltose of 29.21mM (10g/L). An unstructured mathematical model was established for the bioprocess simulation.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus amylovorus | Increased Production of Acetate | Neutral | Small |
| Lactobacillus amylovorus | Reduced D-Lactate Production | Neutral | Small |