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Production of Xylooligosaccharides (XOSs) with Anti-inflammatory and Prebiotic Activities Using Recombinant Endo-1,4-β-xylanases from Bacillus subtilis.

  • 2026-03-16
  • Journal of agricultural and food chemistry 74(11)
    • Nguyen Cao Cuong
    • Dietmar Haltrich
    • Apichat Boontawan
    • Montarop Yamabhai

Study Design

Population
human monocytes
Methods
Two forms of recombinant Bacillus subtilis xylanases, MBP-BsXynA11 and BsXynA11-His, were investigated for their ability to convert xylan into value-added XOS; optimal hydrolysis of xylan was assessed and XOS generated from MBP-BsXynA11 were tested in human monocytes.
  • Rigorous Journal
The enzymatic production of xylooligosaccharides (XOSs) from xylan-rich agricultural byproducts has gained attention as a sustainable and environmentally friendly approach. In this work, two forms of recombinant Bacillus subtilis xylanases, MBP-BsXynA11 (a fusion of a maltose-binding protein with the xylanase) and BsXynA11-His (the xylanase with a 10xHis-tag), were investigated for their ability to convert xylan into value-added XOS. Both enzymes exhibited similar temperature and pH optima at 60 °C and 6.5, with specific activities of 7,900 and 4,800 U/nmol, respectively. However, MBP-BsXynA11 demonstrated higher yields upon recombinant expression, as well as greater specificity and thermostability compared to BsXynA11-His. Optimal hydrolysis of xylan yielded 53% and 51% XOS for MBP-BsXynA11 and BsXynA11-His, respectively. The resulting XOS were water-soluble, with over 90% of the products consisting of xylobiose, xylotriose, and xylotetraose, while xylose accounted for less than 0.5%. Finally, XOS generated from MBP-BsXynA11 demonstrated both prebiotic and anti-inflammatory activities in human monocytes.

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