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Prophylactic effect of Lactobacillus plantarum KSFY06 on HCl/ethanol-induced gastric injury in mice.

  • 2020
  • Food & Function 11(3)
    • Ranran Wang
    • Xiaofei Zeng
    • Bihui Liu
    • Ruokun Yi
    • Xianrong Zhou
    • Jianfei Mu
    • Xin Zhao

Abstract

The present study was conducted to determine the prophylactic effect of Lactobacillus plantarum KSFY06 (LP-KSFY06) on HCl/ethanol-induced gastric injury in Kunming mice. The experimental mice were allocated into six groups: the normal group, HCl/ethanol treated group, HCl/ethanol + ranitidine treated group, HCl/ethanol + Lactobacillus delbrueckii subsp. Bulgaricus (LB) treated group, HCl/ethanol + low concentration of Lactobacillus plantans KSFY06 (LP-KSFY06-L) treated group, and HCl/ethanol + high concentration of Lactobacillus plantans KSFY06 (LP-KSFY06-H) treated group. The changes in daily body weight and food intake of the mice in the HCl/ethanol + LP-KSFY06-H treated group were the closest to those of the HCl/ethanol + ranitidine treated and normal groups. LP-KSFY06 significantly inhibited the formation of gastric mucosal lesions, reduced the area of gastric lesions, inhibited gastric-juice secretion, and increased pH compared with the HCl/ethanol treated group. After the treatment, the serum interleukin-6 (IL)-6, IL-12, tumor necrosis factor-α (TNF-α), and interferon-γ levels and the gastric-tissue IL-6 and IL-12 levels in the LP-KSFY06 (including LP-KSFY06-L and LP-KSFY06-H) group decreased compared with those in the HCl/ethanol treated group. The level of serum and gastric tissue malondialdehyde was lower and the nitric oxide, total superoxide dismutase, and glutathione activities in the LP-KSFY06 treated mice were higher than those in the HCl/ethanol treated mice. Quantitative polymerase chain reaction analysis and western blot analysis showed that LP-KSFY06 increased the mRNA and protein expression of the epidermal growth factor, epidermal growth factor receptor, vascular endothelial growth factor, inhibitor kappaB-α, neuronal nitric oxide synthase, and endothelial NOS and reduced the mRNA and protein expression of nuclear factor kappaB, inducible NOS, cyclooxygenase-2, TNF-α, and IL-1β in gastric tissues compared with the HCl/ethanol treated mice. These experimental results showed that a high concentration (1.0 × 109 CFU per kg B.W.) of LP-KSFY06 had a stronger effect on preventing gastric injury than a low concentration (1.0 × 108 CFU per kg B.W.) of LP-KSFY06. These results suggest that LP-KSFY06 has a potential probiotic effect in preventing gastric injury.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus Bulgaricus & Lactobacillus PlantarumEnhanced Gastric Protein ProtectionBeneficial
Large
Lactobacillus Bulgaricus & Lactobacillus PlantarumReduced Gastric Mucosal Lesion FormationBeneficial
Large
Lactobacillus Bulgaricus & Lactobacillus PlantarumReduced Inflammatory ResponseBeneficial
Large
Lactobacillus Bulgaricus & Lactobacillus PlantarumReduced Oxidative StressBeneficial
Large
Lactobacillus plantarum Lp-115Reduced Gastric Mucosal Lesion FormationBeneficial
Large
Lactobacillus plantarum Lp-115Reduced Malondialdehyde LevelsBeneficial
Moderate
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