Regulatory mechanism of antioxidant enzyme activity, metabolites, transcription levels and key gene expression in blackberry under high-temperature stress.
- 2026-04
- Plant physiology and biochemistry : PPB 233
- PubMed: 41930494
- DOI: 10.1016/j.plaphy.2026.111243
Study Design
- Population
- seedlings of blackberry cultivar 'Ningzhi 4'
- Methods
- High-temperature stress treatment; physiological, transcriptomic, and metabolomic analyses
- Duration
- 72 h
- Funding
- Unclear
Due to global warming, temperature changes can affect plant physiology, gene expression and metabolism. Under high-temperature conditions, the growth, pollination by bees, and fruit quality of blackberry are strongly affected. Therefore, exploring the physiological, transcriptional and metabolic changes in blackberry leaves under high-temperature conditions is highly important for the cultivation of new cultivars of blackberry that are tolerant to high temperature. In this study, the seedlings of the widely planted blackberry cultivar 'Ningzhi 4' in China were treated at high temperature. Under high-temperature stress the activities of SOD and CAT in blackberry increased at 72 h, and the contents of MDA and H2O2 increased at both 6 h and 72 h. The activities of CAT and SOD activities were significantly positively correlated. GO and KEGG analyses revealed that the amino acid synthesis pathway, amino sugar and nucleotide sugar metabolism, purine metabolism pathway, and ABC transport pathway are closely related to the high temperature stress response of blackberry. Metabolomic analysis revealed that the contents of xanthosine, xanthine, UDP-N-acetylglucosamine, D-mannose 6-phosphate, GDP-alpha-D-glucose, L-threonine, L-glutamine, and L-citrulline metabolites increased significantly in blackberry under high-temperature stress. In terms of gene expression, the expression levels of UDP-arabinopyranose mutase and alpha-1,4-galacturonosyltransferase tended to increase over time, whereas the expression levels of L-arabinokinase, alpha-L-arabinofuranosidase, and xylan 1,4-beta-xylosidase tended to decrease. This study revealed that the key pathways involved in the response of blackberry to high-temperature stress were those involved in sugar and nucleotide sugar metabolism, the biosynthesis of amino acids and purine metabolism. Metabolites such as benzoylaconitine, diosgenin glucoside, and MAG (18:1) may be indicators of high temperature in blackberries.
Research Insights
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