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The effect of Lactobacillus paracasei subsp. paracasei L. casei W8® on blood levels of triacylglycerol is independent of colonisation.

  • 2015-01-01
  • Beneficial Microbes 6(3)
    • A. T. Bjerg
    • M. Sørensen
    • L. Krych
    • L. Hansen
    • A. Astrup
    • M. Kristensen
    • D. Nielsen

Abstract

Gut microbiota (GM) dysbiosis has been linked to obesity and its metabolic complications such as cardiovascular disease (CVD). The risk of developing CVD increases with elevated concentration of serum triacylglycerol (TAG). In a blinded, randomised two-arm parallel human intervention study we have previously found that four weeks of supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® (L. casei W8) compared to placebo reduced the concentration of TAG in 64 young healthy adults, an effect, likely mediated by a decreased stearoyl- CoA desaturase-1 (SCD1) activity. In the present study we analysed faecal samples obtained during the intervention study to investigate whether this effect was related to the ability of L. casei W8 to colonise the human gut after supplementation of L. casei W8 (1010 cfu daily) as determined by qPCR specific for L. paracasei and L. casei (L. casei group); whether L. casei W8 consumption affected GM composition as determined by 16S rRNA gene targeted 454/FLX amplicon sequencing; and whether these changes were associated with changes in TAG concentration and SCD1 activity. Faecal samples were collected at baseline, after four weeks supplementation and two weeks after the supplementation was ended, and fasting blood samples were collected at baseline and after 4 weeks. Four weeks supplementation with L. casei W8 did not affect the overall composition of the GM; however, an increase in the relative abundance of the L. casei group from 8.48×10-6% of the total GM compared to 2.83×10-3% at baseline (P<0.001) was observed. Two weeks after supplementation ended, the relative abundance of the L. casei group was still increased 14 times compared to before the intervention (P<0.01). However, neither the increase in the abundance of the L. casei group nor overall GM composition correlated with changes in blood lipids or SCD1 activity.

Keywords: gut microbiota; probiotic; pyrosequencing; qPCR; triacylglycerol.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus casei subsp. caseiLimited Change in Commensal Gut Microbiota CompositionNeutral
Small
Lactobacillus casei subsp. caseiReduced Serum TriacylglycerolsBeneficial
Moderate
Lactobacillus paracasei DSM 13434Reduced Serum TriacylglycerolsBeneficial
Moderate
Lactobacillus paracasei subsp. paracasei UALs-04Reduced Triglyceride LevelsBeneficial
Moderate
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