The Lack of a COPII Cargo Receptor Erv14 Impacts Physiological Functions of the Vacuole in Saccharomyces cerevisiae.

2026-04-23
Traffic (Copenhagen, Denmark) 27(2)
- Paul Rosas-Santiago
- Jorge Luis Ruiz Salas
- Elizabeth Cordoba
- Francisco Vera-López Portillo
- Prisciluis Caheri Salas-Navarrete
- Jaime Arturo Pimentel Cabrera
- Alfredo Martínez
- Nayeli Imelda Roldan Ramírez
- Dieter Chavelas Hernández
- Andrés Saralegui-Amaro
- Martha Calahorra
- Hana Sychrová
- Olga Zimmermannová

PubMed: 42026727
DOI: 10.1111/tra.70035

Study Design

Methods: deletion of the ERV14 gene; comparing gene expression, proteome analysis, and structural modeling

Erv14 is a cargo receptor of COPII vesicles, which is necessary for the efficient trafficking of various membrane proteins. In this work, we demonstrate that the deletion of the ERV14 gene impacts various physiological functions of the vacuole. Compared to the wild-type cells, cells lacking the ERV14 gene exhibited higher vacuolar pH and altered vacuolar morphology with increased fragmentation. In addition, erv14Δ cells exhibit a thinner cell wall and an impaired process of endocytosis. We also found the importance of ERV14 for cells to overcome environmental stresses, such as neutral external pH, increased zinc and calcium concentrations, and high temperature. Furthermore, comparing gene expression, proteome analysis, and structural modeling revealed new interactions between Erv14 and several vacuolar proteins, including subunits of V-ATPase and other proteins involved in carbon metabolism.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Saccharomyces cerevisiae	—	Impaired Vacuolar Function	Harmful	Moderate	View source the deletion of the ERV14 gene impacts various physiological functions of the vacuole. Compared to the wild-type cells, cells lacking the ERV14 gene exhibited higher vacuolar pH and altered vacuolar morphology with increased fragmentation.
Saccharomyces cerevisiae	—	Improved Stress Tolerance	Harmful	Moderate	View source we also found the importance of ERV14 for cells to overcome environmental stresses, such as neutral external pH, increased zinc and calcium concentrations, and high temperature.