The probiotic Lactobacillus fermentum 296 attenuates cardiometabolic disorders in high fat diet-treated rats.

2019-12
Nutrition, Metabolism and Cardiovascular Diseases 29(12)
- R. G. S. Cavalcante
- Thatyane Mariano Rodrigues de Albuquerque
- M. O. de Luna Freire
- Georgianna de Araújo Henriques Ferreira
- L. A. Carneiro dos Santos
- M. Magnani
- J. Cruz
- V. Braga
- E. L. de Souza
- J. L. de Brito Alves

PubMed: 31640890
DOI: 10.1016/J.NUMECD.2019.08.003

Study Design

Population: Male Wistar rats
Methods: Controlled experimental study

Abstract

Background and aim: High-fat (HF) diet consumption has been associated with gut dysbiosis and increased risk of dyslipidemia, type 2 diabetes mellitus and hypertension. Probiotic administration has been suggested as a safe therapeutic strategy for the treatment of cardiometabolic disorders. This study was designed to assess the effects of probiotic Lactobacillus (L.) fermentum 296, a fruit-derived bacteria strain, against cardiometabolic disorders induced by HF diet.

Methods and results: Male Wistar rats were divided into control diet (CTL); HF diet; and HF diet treated with Lactobacillus fermentum 296 (HF + Lf 296). The L. fermentum 296 strain at 1 × 109 colony forming units (CFU)/ml were daily administered by oral gavage for 4 weeks. The results showed that rats fed with HF diet displayed insulin resistance, reduced Lactobacillus spp. counts in feces, serum lipids, and oxidative profile. Rats fed on HF diet also demonstrated augmented blood pressure associated with sympathetic hyperactivity and impaired baroreflex control. The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure in HF rats without affecting baroreflex sensibility.

Conclusion: Our results suggest the ability of L. fermentum 296 improve biochemical and cardiovascular parameters altered in cardiometabolic disorders.

Keywords: Dyslipidemia; Hypertension; Lactobacillus fermentum; Microbiota; Probiotics.

Research Insights

Lactobacillus fermentum LF61→Improved Lactobacillus Counts
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum LF61→Reduced Hyperlipidemia Symptoms
The administration of L. fermentum 296 for 4 weeks...alleviated hyperlipidemia.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum LF61→Reduced Sympathetic Hyperactivity
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum LF61→Reduced Systolic Blood Pressure
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum MAK20L13F→Improved Insulin Sensitivity
The results showed that rats fed with HF diet displayed insulin resistance... The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum MAK20L13F→Improved Lipid Profile
The administration of L. fermentum 296 for 4 weeks... alleviated hyperlipidemia...
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum MAK20L13F→Reduced Sympathetic Hyperactivity
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum MAK20L13F→Reduced Systolic Blood Pressure
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum UALf-32→Improved Insulin Sensitivity
The results showed that rats fed with HF diet displayed insulin resistance... The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum UALf-32→Improved Lactobacillus Counts
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum UALf-32→Reduced Blood Lipid Levels
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum UALf-32→Reduced Sympathetic Hyperactivity
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum UALf-32→Reduced Systolic Blood Pressure
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum VPro 14→Improved Insulin Sensitivity
The results showed that rats fed with HF diet displayed insulin resistance... The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum VPro 14→Reduced Blood Lipid Levels
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum VPro 14→Reduced Sympathetic Hyperactivity
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum VPro 14→Reduced Systolic Blood Pressure
The administration of L. fermentum 296 for 4 weeks recovered fecal Lactobacillus sp. counts and alleviated hyperlipidemia, sympathetic hyperactivity, and reduced systolic blood pressure.
Effect
Beneficial
Effect size
Moderate