Three Strains of Lactobacillus Derived from Piglets Alleviated Intestinal Oxidative Stress Induced by Diquat through Extracellular Vesicles.

2023-09-28
Nutrients 15(19)

PubMed: 37836484
DOI: 10.3390/nu15194198

Study Design

Population: Six Lactobacillus strains isolated from the intestines of neonatal piglets fed with PCPs
Methods: Six Lactobacillus strains isolated from the intestines of neonatal piglets fed with PCPs were studied to investigate the relationship between Lactobacillus and intestinal oxidative stress; subsequently, the extracellular vesicles (EVs) of these three Lactobacillus strains were extracted to verify their intestinal protection mode of action

Rigorous Journal
Animal Study

Previous studies found that Poria cocos polysaccharides (PCPs) significantly enhanced the antioxidant activity in piglet intestines while increasing the abundance of Lactobacillus. However, the relationship between Lactobacillus and antioxidant activity has yet to be verified, and the mode of action needs further investigation. Six Lactobacillus strains isolated from the intestines of neonatal piglets fed with PCPs were studied to investigate the relationship between Lactobacillus and intestinal oxidative stress. The results showed that three of them alleviated intestinal oxidative stress and protected the intestinal barrier. Subsequently, we extracted the extracellular vesicles (EVs) of these three Lactobacillus strains to verify their intestinal protection mode of action. We found that these EVs exerted an excellent antioxidant effect and intestinal barrier protection and could directly improve intestinal microbial composition. Our findings suggested that the EVs of the three Lactobacillus strains could enhance antioxidant activity by improving the physical intestinal barrier and remodeling gut microbiota. Unlike probiotics, which should be pre-colonized, EVs can act directly on the intestines. This study provides new ideas for the subsequent development of products to protect intestinal health.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus amylovorus	—	Improved Gut Microbiota Composition	Beneficial	Small	View source could directly improve intestinal microbial composition
Lactobacillus amylovorus	—	Improved Intestinal Barrier Function	Beneficial	Moderate	View source three of them alleviated intestinal oxidative stress and protected the intestinal barrier
Lactobacillus amylovorus	—	Reduced Oxidative Stress in Intestinal Epithelial Cells	Beneficial	Moderate	View source three of them alleviated intestinal oxidative stress and protected the intestinal barrier