- 2026-04
- Phytomedicine : international journal of phytotherapy and phytopharmacology 153
- Xinrong Tian
- Yan Huang
- Chenxin He
- Ertong Dai
- Xiwen Zhang
- Peng Zou
- Ruiyan Liu
- Minlong Xia
- Zixuan Lu
- Bilin Jin
- Siqi Wang
- Yue Zhang
- Hui Kong
- Huihua Qu
- Yan Zhao
Study Design
- Methods
- IF+Zn-CDs were synthesized via bottom-up using the two precursors, characterized by TEM, FTIR, and XPS. An IMQ-induced model evaluated in vivo efficacy; transcriptomic analysis identified modulated pathways, and ROS/intracellular calcium levels clarified NLRP3 regulation, with pyroptosis and MAPK/NF-κB effects validated.
- Funding
- Unclear
Background
Psoriasis is a chronic inflammatory cutaneous disorder characterized by immune dysregulation and aberrant keratinocyte proliferation, necessitating novel effective anti-inflammatory therapies.Purpose
This study synthesized zinc-doped carbon dots (IF+Zn-CDs) from Isatis indigotica Folium and calamine, investigating their therapeutic potential and underlying molecular mechanisms in psoriasis treatment.Study design
A bottom-up approach prepared IF+Zn-CDs for systematic physicochemical characterization. Their efficacy was assessed in an IMQ-induced murine psoriasis model, with anti-inflammatory mechanisms explored via transcriptomic and mechanistic assays.Methods
IF+Zn-CDs were synthesized via bottom-up using the two precursors, characterized by TEM, FTIR, and XPS. An IMQ-induced model evaluated in vivo efficacy; transcriptomic analysis identified modulated pathways, and ROS/intracellular calcium levels clarified NLRP3 regulation, with pyroptosis and MAPK/NF-κB effects validated.Results
The as-synthesized IF+Zn-CDs exhibited a uniform size distribution, excellent dispersibility, high crystallinity, and superior biocompatibility. In the IMQ-induced murine model of psoriasis, IF+Zn-CDs markedly attenuated psoriatic manifestations including erythema, scaling, epidermal hyperplasia, and inflammatory cell infiltration. Transcriptomic analysis revealed that IF+Zn-CDs modulate multiple signaling pathways associated with psoriatic inflammation. Mechanistic investigations confirmed that IF+Zn-CDs inhibit NLRP3 inflammasome activation through the reduction of ROS production and intracellular calcium levels, and further suppress pyroptosis while blocking the MAPK/NF-κB signaling pathway, ultimately ameliorating psoriasiform dermatitis.Conclusion
IF+Zn-CDs have potent anti-inflammatory activities and ameliorate psoriasiform dermatitis, highlighting their promising potential as novel anti-inflammatory agents for psoriasis.