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A Synbiotic Combining Chitin-Glucan and Lactobacillus acidophilus NCFM Induces a Colonic Molecular Signature Soothing Intestinal Pain and Inflammation in an Animal Model of IBS.

  • 2024-10-05
  • International journal of molecular sciences 25(19)
    • Lena Capirchio
    • Christel Rousseaux
    • Caroline Dubuquoy
    • Arthur C Ouwehand
    • Véronique Maquet
    • Salvatore Modica
    • Edouard Louis
    • Pierre Desreumaux
    • Jan Tack

Study Design

Population
a rat model of long-lasting colon hypersensitivity
Methods
the intracolonic pressure was assessed during the 9-week experiment in animals receiving CG in association or not with NCFM and compared to that in Lacticaseibacillus paracasei Lpc-37®-treated animals and control rats receiving tap water; the effects of the synbiotic were evaluated using the Wallace score, the quantification of colon myeloperoxidase (MPO) and the master genes driving analgesia and inflammation
  • Rigorous Journal
  • Animal Study
Chitin-glucan (CG) is a new generation of prebiotic. Lactobacillus acidophilus NCFM® (NCFM) is a probiotic with the ability to decrease abdominal pain. We evaluate the functional and molecular gastrointestinal responses to a synbiotic administration combining CG and NCFM in a rat model of long-lasting colon hypersensitivity. The intracolonic pressure was assessed during the 9-week experiment in animals receiving CG in association or not with NCFM and compared to that in Lacticaseibacillus paracasei Lpc-37®-treated animals and control rats receiving tap water. The effects of the synbiotic were evaluated using the Wallace score, the quantification of colon myeloperoxidase (MPO) and the master genes driving analgesia and inflammation. CG 1.5 alone and NCFM 109 colony forming units (CFU) alone similarly decreased the visceral pain sensitivity. Lpc-37 had no significant effect. The best profile of pain perception inhibition was obtained with the combination of CG 1.5 g and NCFM 109 CFU, confirming a synbiotic property. This synbiotic treatment significantly reduced macroscopic colonic lesions and MPO concentrations, and induced master genes involved in analgesia (CB1, CB2, MOR, PPARα), with a downregulation of inflammatory cytokines (IL-1β, TNFα) and an induction of IL-10 and PPARγ. In conclusion, CG 1.5 g + NCFM 109 CFU significantly decreased visceral pain perception and intestinal inflammation through the regulation of master genes.

Research Insights

  • Lactobacillus acidophilus NCFM→Reduced Intestinal Inflammation

    This synbiotic treatment significantly reduced macroscopic colonic lesions and MPO concentrations, and induced master genes involved in analgesia (CB1, CB2, MOR, PPARα), with a downregulation of inflammatory cytokines (IL-1β, TNFα) and an induction of IL-10 and PPARγ.

    Effect
    Beneficial
    Effect size
    Moderate
  • Lactobacillus acidophilus NCFM→Reduced Visceral Hypersensitivity

    CG 1.5 alone and NCFM 10^9 colony forming units (CFU) alone similarly decreased the visceral pain sensitivity. The best profile of pain perception inhibition was obtained with the combination of CG 1.5 g and NCFM 10^9 CFU

    Effect
    Beneficial
    Effect size
    Moderate
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