Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients
- 2020-09-22
- PLOS ONE 15(9)
- M. Invernici
- F. Furlaneto
- S. Salvador
- A. Ouwehand
- S. Salminen
- Anastasia Mantziari
- G. Vinderola
- E. Ervolino
- S. Santana
- P. Silva
- M. Messora
- PubMed: 32960889
- DOI: 10.1371/journal.pone.0238425
Abstract
Objective: To evaluate the effects of Bifidobacterium animalis subsp. lactis HN019 (HN019) on clinical periodontal parameters (plaque accumulation and gingival bleeding), on immunocompetence of gingival tissues [expression of beta-defensin (BD)-3, toll-like receptor 4 (TLR4), cluster of differentiation(CD)-57 and CD-4], and on immunological properties of saliva (IgA levels) in non-surgical periodontal therapy in generalized chronic periodontitis (GCP) patients. Adhesion to buccal epithelial cells (BEC) and the antimicrobial properties of HN019 were also investigated.
Materials and methods: Thirty patients were recruited and monitored clinically at baseline (before scaling and root planing-SRP) and after 30 and 90 days. Patients were randomly assigned to Test (SRP+Probiotic, n = 15) or Control (SRP+Placebo, n = 15) group. Probiotic lozenges were used for 30 days. Gingival tissues and saliva were immunologically analyzed. The adhesion of HN019 with or without Porphyromonas gingivalis in BEC and its antimicrobial properties were investigated in in vitro assays. Data were statistically analyzed (p<0.05).
Results: Test group presented lower plaque index (30 days) and lower marginal gingival bleeding (90 days) when compared with Control group. Higher BD-3, TLR4 and CD-4 expressions were observed in gingival tissues in Test group than in Control group. HN019 reduced the adhesion of P. gingivalis to BEC and showed antimicrobial potential against periodontopathogens.
Conclusion: Immunological and antimicrobial properties of B. lactis HN019 make it a potential probiotic to be used in non-surgical periodontal therapy of patients with GCP.
Clinical relevance: B. lactis HN019 may be a potential probiotic to improve the effects of non-surgical periodontal therapy. Name of the registry and registration number (ClinicalTrials.gov): "Effects of probiotic therapy in the treatment of periodontitis"-NCT03408548.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Bifidobacterium animalis | Improved Immunological Response in Gingival Tissues | Beneficial | Moderate |
| Bifidobacterium animalis | Improved Periodontal Health | Beneficial | Moderate |
| Bifidobacterium animalis | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium animalis | Reduced Pathogen Adhesion to Buccal Epithelial Cells | Beneficial | Moderate |
| Bifidobacterium animalis | Reduced Plaque Accumulation | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis | Improved Immunological Response in Gingival Tissues | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis | Reduced Pathogen Adhesion to Buccal Epithelial Cells | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis | Reduced Plaque Accumulation | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Enhanced Immunological Marker Expression | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis B420 | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Reduced Gingivalis Adhesion | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Reduced Plaque Index | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Increased CD-4 Expression | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis BLC1 | Increased Expression of Beta-Defensin 3 | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis BLC1 | Increased Expression of Toll-like Receptor 4 (TLR4) | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis BLC1 | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Reduced Periodontopathogen Activity | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis BLC1 | Reduced Plaque Accumulation | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Reduced Porphyromonas Gingivalis Adhesion to Buccal Epithelial Cells | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Increased CD-4 Expression | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis BS-01 | Increased Expression of Beta-Defensin 3 | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis BS-01 | Increased Expression of Toll-like Receptor 4 (TLR4) | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis BS-01 | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Reduced Pathogen Adhesion to Buccal Epithelial Cells | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Reduced Plaque Accumulation | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Reduced Pathogen Adhesion to Buccal Epithelial Cells | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Reduced Periodontal Pathogens | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Reduced Plaque Accumulation | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis HN019 | Increased CD-4 Expression | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis HN019 | Increased Expression of Beta-Defensin 3 | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis HN019 | Increased Expression of Toll-like Receptor 4 (TLR4) | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis HN019 | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis HN019 | Reduced Periodontopathogen Activity | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis HN019 | Reduced Plaque Accumulation | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis HN019 | Reduced Porphyromonas Gingivalis Adhesion to Buccal Epithelial Cells | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Increased CD-4 Expression | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Increased Expression of Beta-Defensin 3 | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Increased Expression of Toll-like Receptor 4 (TLR4) | Beneficial | Small |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Reduced Periodontopathogen Activity | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Reduced Plaque Accumulation | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Reduced Porphyromonas Gingivalis Adhesion to Buccal Epithelial Cells | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis VK2 | Elevated Immune Marker Expression | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis VK2 | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis VK2 | Reduced Gingivalis Adhesion | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis VK2 | Reduced Plaque Accumulation | Beneficial | Moderate |
| Bifidobacterium lactis | Improved Immunocompetence of Gingival Tissues | Beneficial | Large |
| Bifidobacterium lactis | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium lactis | Reduced Pathogen Adhesion to Buccal Epithelial Cells | Beneficial | Moderate |
| Bifidobacterium lactis | Reduced Periodontopathogen Activity | Beneficial | Large |
| Bifidobacterium lactis | Reduced Plaque Accumulation | Beneficial | Moderate |
| Bifidobacterium lactis HN019 | Enhanced CD4 Expression | Beneficial | Moderate |
| Bifidobacterium lactis HN019 | Enhanced TLR4 Expression | Beneficial | Moderate |
| Bifidobacterium lactis HN019 | Increased Expression of Beta-Defensin 3 | Beneficial | Small |
| Bifidobacterium lactis HN019 | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium lactis HN019 | Reduced Plaque Accumulation | Beneficial | Moderate |
| Bifidobacterium lactis HN019 | Reduced Porphyromonas Gingivalis Adhesion to Buccal Epithelial Cells | Beneficial | Moderate |
| Bifidobacterium lactis SD-5219 | Improved Immunocompetence of Gingival Tissues | Beneficial | Large |
| Bifidobacterium lactis SD-5219 | Reduced Gingival Bleeding | Beneficial | Moderate |
| Bifidobacterium lactis SD-5219 | Reduced Pathogen Adhesion | Beneficial | Moderate |
| Bifidobacterium lactis SD-5219 | Reduced Plaque Accumulation | Beneficial | Moderate |