Correlation of changes in HIF-1α and p53 expressions with vitamin B3 deficiency in skin cancer patients.

2019-10
Giornale italiano di dermatologia e venereologia : organo ufficiale, Societa italiana di dermatologia e sifilografia 154(5)
- Ting Liu
- Hao Yang
- Yunzhu Mou
- Hao Zhang

PubMed: 29064206
DOI: 10.23736/s0392-0488.17.05775-3

Study Design

Type: Randomized Controlled Trial (RCT)
Population: 20 non-melanoma skin cancer patients with positive HIF-1α and p53 expressions
Methods: randomly divided into two groups, oral administration of 500 mg vitamin B3 every day for 1 week
Duration: 1 week
Funding: Unclear

Background

To investigate the correlation of changes in hypoxia-inducible factor-1α (HIF-1α) and p53 expressions with vitamin B3 deficiency in skin cancer patients.

Methods

Twenty non-melanoma skin cancer patients with positive HIF-1α and p53 expressions were selected and randomly divided into two groups, the placebo group and the experimental group. After an appropriate number of cancer tissues were taken, the experimental group was treated with oral administration of 500 mg vitamin B3 every day, while the placebo group was treated with oral administration of the same amount of placebo; after 1 week, the skin cancer tissues in the same part were taken, and the skin tissues of healthy people were taken as the control group; the mRNA and protein expression levels of HIF-1α and p53 in tissues were detected.

Results

HIF-1α and p53 were mainly expressed in the nucleus in non-melanoma skin cancer. The protein and mRNA expression levels of HIF-1α and p53 in tissues of skin cancer patients were significantly increased compared with those in skin tissues of healthy people (P<0.05). The protein and mRNA expression levels of HIF-1α and p53 in tissues of skin cancer patients were significantly decreased at 1 week after the oral administration of vitamin B3 compared with those before the oral administration of vitamin B3 (P<0.05), but the protein and mRNA expression levels of HIF-1α and p53 in tissues of skin cancer patients in placebo group had no significant changes (P>0.05). The vitamin B3 deficiency in skin cancer patients was positively correlated with the expressions of HIF-1α and p53.

Conclusions

The expression levels of HIF-1α and p53 in tissues of skin cancer patients are significantly increased compared with those in skin tissues of healthy people, and the changes in their expressions are positively correlated with the vitamin B3 deficiency. Supplementing vitamin B3 has a certain protective effect on skin cancer patients.

Research Insights

Vitamin B3→Reduced HIF-1α Protein Level
The protein and mRNA expression levels of HIF-1α and p53 in tissues of skin cancer patients were significantly decreased at 1 week after the oral administration of vitamin B3 compared with those before the oral administration of vitamin B3 (P<0.05)
Effect
Beneficial
Effect size
Moderate
Dose
500 mg/day
Vitamin B3→Reduced HIF-1α mRNA Expression
The protein and mRNA expression levels of HIF-1α and p53 in tissues of skin cancer patients were significantly decreased at 1 week after the oral administration of vitamin B3 compared with those before the oral administration of vitamin B3 (P<0.05)
Effect
Beneficial
Effect size
Moderate
Dose
500 mg/day
Vitamin B3→Reduced p53 Protein Expression
The protein and mRNA expression levels of HIF-1α and p53 in tissues of skin cancer patients were significantly decreased at 1 week after the oral administration of vitamin B3 compared with those before the oral administration of vitamin B3 (P<0.05)
Effect
Beneficial
Effect size
Moderate
Dose
500 mg/day
Vitamin B3→Reduced p53 mRNA Expression
The protein and mRNA expression levels of HIF-1α and p53 in tissues of skin cancer patients were significantly decreased at 1 week after the oral administration of vitamin B3 compared with those before the oral administration of vitamin B3 (P<0.05)
Effect
Beneficial
Effect size
Moderate
Dose
500 mg/day