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Effects of Lactobacillus acidophilus KLDS1.0901 on Proliferation and Apoptosis of Colon Cancer Cells

  • 2022-02-11
  • Frontiers in Microbiology 12
    • Yingxue Yue
    • Song Wang
    • Jialu Shi
    • Qinggang Xie
    • Na Li
    • Jiaqi Guan
    • S. Evivie
    • Fei Liu
    • Bailiang Li
    • G. Huo

Abstract

Colon cancer is the most common type of malignant tumor. The cytotoxicity effect of lactic acid bacteria may be active by inhibiting cancer cell proliferation, producing anticancer compounds, and inducing apoptosis in cancer cells, but the mechanism is unclear. Our previous study revealed that Lactobacillus acidophilus KLDS1.0901 has good probiotic properties. In this study, We screened out the highest inhibition rate of L. acidophilus KLDS1.0901 and assessed the effects on the proliferation of HT-29, Caco-2, and IEC-6 cells. Then, the apoptosis mechanism of HT-29 cells was studied when treated with L. acidophilus KLDS1.0901. Results showed that L. acidophilus KLDS1.0901 inhibited the proliferation of HT-29 and Caco-2 cells in a dose-dependent manner and reached the maximum under the condition of multiplicity of infection (MOI) = 100 (rate of Lactobacillus to cells) at 48 h. With the increase in time and MOI, reactive oxygen species in HT-29 cells, the apoptosis rates of HT-29 cells were increased, and the amount of blue fluorescence of the cells was also increased after Hoechst 33258 staining. Furthermore, L. acidophilus KLDS1.0901 reduced the mitochondrial membrane potential of HT-29 cells. Notably, 1,133 differentially expressed genes were screened by transcriptomics research, including 531 up-regulated genes and 602 down-regulated genes. These genes were involved in the nuclear factor κB and PI3K-AKT signaling pathways related to the apoptosis of HT-29 cells. These findings suggested that L. acidophilus KLDS1.0901 has the potential to be used in the development of a new type of functional foods for adjuvant treatment of colon cancer.

Keywords: HT-29 cell; Lactobacillus acidophilus; apoptosis; colon cancer; proliferation.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus acidophilusIncreased Apoptosis in Colon Cancer CellsBeneficial
Moderate
Lactobacillus acidophilusModulation of Gene Expression in Colon Cancer Cell ApoptosisBeneficial
Moderate
Lactobacillus acidophilusReduced Colon Cancer Cell ProliferationBeneficial
Moderate
Lactobacillus acidophilusReduced Mitochondrial Membrane Potential in Colon Cancer CellsBeneficial
Moderate
Lactobacillus acidophilus UALa01Altered Apoptosis Pathway Gene ExpressionBeneficial
Large
Lactobacillus acidophilus UALa01Increased Apoptosis in Colon Cancer CellsBeneficial
Moderate
Lactobacillus acidophilus UALa01Reduced Colon Cancer Cell ProliferationBeneficial
Moderate
Lactobacillus acidophilus UALa01Reduced Mitochondrial Membrane Potential in Colon Cancer CellsBeneficial
Moderate

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