Effects of skim milk fermented with Lactobacillus plantarum WW on the constitutions of rats fed a high-fat diet.

2020-06
Journal of Dairy Science 103(6)
- Yang Li
- Junrui Wu
- Chengxu Cao
- Xinyuan Zhu
- Xueting Sun
- R. Wu

PubMed: 32278555
DOI: 10.3168/jds.2019-17560

Study Design

Type: Randomized Controlled Trial (RCT)
Population: Sprague-Dawley rats
Methods: Controlled experimental study

Rigorous Journal
Animal Study

Abstract

This study investigated the effects of Lactobacillus plantarum WW-fermented skim milk (FSM) on the physiques of rats fed a high-fat diet and the mechanism of lipid lowering. Sprague-Dawley rats were randomly divided into a normal diet group (A), a high-fat diet group (B), a skim milk diet group (C), and an L. plantarum WW FSM diet group (D). After 12-wk feeding, we found that treatment with L. plantarum WW FSM could significantly alleviate symptoms in the pathological group. Meanwhile, high-throughput sequencing analysis showed that L. plantarum WW FSM also had a certain regulatory effect on the intestinal microorganisms in rats, which can increase the number of lactic acid bacteria and Bacteroides in the intestine. More importantly, real-time quantitative PCR and Western blot analysis showed that the probiotic was also involved in the expression of genes related to fat metabolism, especially the PPARB and CEBPB genes. Our study supports the hypothesis that the WW strain of L. plantarum could be a potential probiotic to be used in functional foods to alter lipid metabolism and reduce cholesterol levels.

Keywords: Lactobacillus plantarum WW; high-fat diet; hyperlipidemia; lipid metabolism.

Research Insights

Lactobacillus plantarum DR7→Improved Lipid Metabolism
real-time quantitative PCR and Western blot analysis showed that the probiotic was also involved in the expression of genes related to fat metabolism, especially the PPARB and CEBPB genes.
Effect
Beneficial
Effect size
Large
Lactobacillus plantarum DR7→Increased Lactic Acid Bacteria Levels
high-throughput sequencing analysis showed that L. plantarum WW FSM also had a certain regulatory effect on the intestinal microorganisms in rats, which can increase the number of lactic acid bacteria and Bacteroides in the intestine.
Effect
Beneficial
Effect size
Moderate
Lactobacillus plantarum DR7→Reduced Pathological Symptoms
After 12-wk feeding, we found that treatment with L. plantarum WW FSM could significantly alleviate symptoms in the pathological group.
Effect
Beneficial
Effect size
Large
Lactobacillus plantarum DR7→Reduced Total Cholesterol Levels
Our study supports the hypothesis that the WW strain of L. plantarum could be a potential probiotic to be used in functional foods to alter lipid metabolism and reduce cholesterol levels.
Effect
Beneficial
Effect size
Large
Lactobacillus plantarum UALp-05M→Improved Lipid Metabolism
real-time quantitative PCR and Western blot analysis showed that the probiotic was also involved in the expression of genes related to fat metabolism, especially the PPARB and CEBPB genes.
Effect
Beneficial
Effect size
Large
Lactobacillus plantarum UALp-05M→Increased Beneficial Gut Microbiota
high-throughput sequencing analysis showed that L. plantarum WW FSM also had a certain regulatory effect on the intestinal microorganisms in rats, which can increase the number of lactic acid bacteria and Bacteroides in the intestine.
Effect
Beneficial
Effect size
Moderate
Lactobacillus plantarum UALp-05M→Reduced Symptoms Associated with High-Fat Diet
we found that treatment with L. plantarum WW FSM could significantly alleviate symptoms in the pathological group.
Effect
Beneficial
Effect size
Moderate
Lactobacillus plantarum UALp-05M→Reduced Total Cholesterol Levels
Our study supports the hypothesis that the WW strain of L. plantarum could be a potential probiotic to be used in functional foods to alter lipid metabolism and reduce cholesterol levels.
Effect
Beneficial
Effect size
Large