Effects of skim milk fermented with Lactobacillus plantarum WW on the constitutions of rats fed a high-fat diet.
- 2020-06
- Journal of Dairy Science 103(6)
- Yang Li
- Junrui Wu
- Chengxu Cao
- Xinyuan Zhu
- Xueting Sun
- R. Wu
- PubMed: 32278555
- DOI: 10.3168/jds.2019-17560
Abstract
This study investigated the effects of Lactobacillus plantarum WW-fermented skim milk (FSM) on the physiques of rats fed a high-fat diet and the mechanism of lipid lowering. Sprague-Dawley rats were randomly divided into a normal diet group (A), a high-fat diet group (B), a skim milk diet group (C), and an L. plantarum WW FSM diet group (D). After 12-wk feeding, we found that treatment with L. plantarum WW FSM could significantly alleviate symptoms in the pathological group. Meanwhile, high-throughput sequencing analysis showed that L. plantarum WW FSM also had a certain regulatory effect on the intestinal microorganisms in rats, which can increase the number of lactic acid bacteria and Bacteroides in the intestine. More importantly, real-time quantitative PCR and Western blot analysis showed that the probiotic was also involved in the expression of genes related to fat metabolism, especially the PPARB and CEBPB genes. Our study supports the hypothesis that the WW strain of L. plantarum could be a potential probiotic to be used in functional foods to alter lipid metabolism and reduce cholesterol levels.
Keywords: Lactobacillus plantarum WW; high-fat diet; hyperlipidemia; lipid metabolism.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Altered Lipid Metabolism | Beneficial | Large |
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus Bulgaricus & Lactobacillus Plantarum | Reduced Symptoms Associated with High-Fat Diet | Beneficial | Moderate |
| Lactobacillus plantarum | Altered Lipid Metabolism | Beneficial | Large |
| Lactobacillus plantarum | Reduced Symptoms Associated with High-Fat Diet | Beneficial | Moderate |
| Lactobacillus plantarum | Reduced Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum 14D | Altered Lipid Metabolism | Beneficial | Large |
| Lactobacillus plantarum 14D | Reduced Pathological Symptoms | Beneficial | Large |
| Lactobacillus plantarum 14D | Reduced Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum 14D | Regulated Gut Microbiota | Beneficial | Moderate |
| Lactobacillus plantarum DR7 | Improved Lipid Metabolism | Beneficial | Large |
| Lactobacillus plantarum DR7 | Increased Lactic Acid Bacteria Levels | Beneficial | Moderate |
| Lactobacillus plantarum DR7 | Reduced Pathological Symptoms | Beneficial | Large |
| Lactobacillus plantarum DR7 | Reduced Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum DSM 6595 | Improved Fat Metabolism Gene Expression | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6595 | Improved Gut Microbiota Composition | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6595 | Reduced Hyperlipidemia Symptoms | Beneficial | Large |
| Lactobacillus plantarum DSM 6595 | Reduced Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum DSM 6596 | Improved Intestinal Microbial Composition | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Improved Lipid Metabolism | Beneficial | Large |
| Lactobacillus plantarum DSM 6596 | Reduced Symptoms Associated with High-Fat Diet | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Reduced Hyperlipidemia Symptoms | Beneficial | Large |
| Lactobacillus plantarum L-137 | Regulated Intestinal Microorganisms | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Altered Lipid Metabolism | Beneficial | Large |
| Lactobacillus plantarum LP-01 | Increased Beneficial Intestinal Microorganisms | Beneficial | Moderate |
| Lactobacillus plantarum LP-01 | Reduced Hyperlipidemia Symptoms | Beneficial | Large |
| Lactobacillus plantarum Lp-115 | Enhanced Beneficial Gut Microbiota | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Improved Fat Metabolism Gene Expression | Beneficial | Moderate |
| Lactobacillus plantarum Lp-115 | Improved Lipid Metabolism | Beneficial | Large |
| Lactobacillus plantarum Lp-115 | Reduced Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum LP09 | Altered Lipid Metabolism | Beneficial | Large |
| Lactobacillus plantarum LP09 | Increased Autochthonous Lactic Acid Bacteria Levels | Beneficial | Moderate |
| Lactobacillus plantarum LP09 | Increased Bacteroides Levels in the Intestine | Beneficial | Moderate |
| Lactobacillus plantarum LP09 | Reduced Hyperlipidemia Symptoms | Beneficial | Large |
| Lactobacillus plantarum LP09 | Reduced Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum N13 | Altered Lipid Metabolism | Beneficial | Large |
| Lactobacillus plantarum N13 | Reduced Pathological Symptoms | Beneficial | Large |
| Lactobacillus plantarum N13 | Regulated Intestinal Microorganisms | Beneficial | Moderate |
| Lactobacillus plantarum Rosell-1012 | Enhanced Fat Metabolism Gene Expression | Beneficial | Large |
| Lactobacillus plantarum Rosell-1012 | Reduced Symptoms of High-Fat Diet Pathologies | Beneficial | Large |
| Lactobacillus plantarum Rosell-1012 | Regulated Intestinal Microorganisms | Beneficial | Moderate |
| Lactobacillus plantarum Rosell-A | Improved Fat Metabolism Regulation | Beneficial | Large |
| Lactobacillus plantarum Rosell-A | Reduced Symptoms Associated with High-Fat Diet | Beneficial | Moderate |
| Lactobacillus plantarum Rosell-A | Reduced Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum Rosell-A | Regulated Gut Microbiota | Beneficial | Moderate |
| Lactobacillus plantarum SD-5209 | Improved Beneficial Intestinal Bacteria | Beneficial | Moderate |
| Lactobacillus plantarum SD-5209 | Reduced Blood Lipid Levels | Beneficial | Large |
| Lactobacillus plantarum SD-5209 | Reduced Pathological Symptoms | Beneficial | Large |
| Lactobacillus plantarum SD5209 | Improved Lipid Metabolism | Beneficial | Large |
| Lactobacillus plantarum SD5209 | Increased Beneficial Gut Microbiota | Beneficial | Moderate |
| Lactobacillus plantarum SD5209 | Reduced Symptoms Associated with High-Fat Diet | Beneficial | Moderate |
| Lactobacillus plantarum SD5209 | Reduced Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum UALp-05 | Enhanced Fat Metabolism Gene Expression | Beneficial | Large |
| Lactobacillus plantarum UALp-05 | Reduced Symptoms Associated with High-Fat Diet | Beneficial | Moderate |
| Lactobacillus plantarum UALp-05M | Improved Lipid Metabolism | Beneficial | Large |
| Lactobacillus plantarum UALp-05M | Increased Beneficial Gut Microbiota | Beneficial | Moderate |
| Lactobacillus plantarum UALp-05M | Reduced Symptoms Associated with High-Fat Diet | Beneficial | Moderate |
| Lactobacillus plantarum UALp-05M | Reduced Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum VPro 10 | Reduced Symptoms Associated with High-Fat Diet | Beneficial | Moderate |
| Lactobacillus plantarum WCFS1 | Improved Fat Metabolism Gene Expression | Beneficial | Moderate |
| Lactobacillus plantarum WCFS1 | Reduced Symptoms of High-Fat Diet Pathologies | Beneficial | Large |
| Lactobacillus plantarum WCFS1 | Reduced Total Cholesterol Levels | Beneficial | Large |
| Lactobacillus plantarum WCFS1 | Regulated Gut Microbiota | Beneficial | Moderate |