Levilactobacillus brevis MG5311 Alleviates Ethanol-Induced Liver Injury by Suppressing Hepatic Oxidative Stress in C57BL/6 Mice.

2022-12-15
Microorganisms 10(12)
- Hyunna Jung
- Sohyeon You
- Soo-Im Choi
- Chang-Ho Kang
- Gun-Hee Kim

PubMed: 36557739
DOI: 10.3390/microorganisms10122488

Study Design

Population: C57BL/6 mice with liver injuries induced by chronic ethanol plus binge feeding
Methods: <i>Levilactobacillus brevis</i> MG5311 was administered orally at a dose of 1 × 10^9 CFU/mouse once daily for 32 days

Alcoholic liver disease (ALD), caused by excessive alcohol consumption, leads to high mortality. We investigated the hepatoprotective effect of Levilactobacillus brevis MG5311 in C57BL/6 mice with liver injuries induced by chronic ethanol plus binge feeding. L. brevis MG5311 was administered orally at a dose of 1 × 10⁹ CFU/mouse once daily for 32 days. L. brevis MG5311 administration significantly reduced serum ALT, AST, and triglyceride (TG) levels in ethanol-fed mice. L. brevis MG5311 also decreased malondialdehyde levels and increased glutathione peroxidase (GPx) activity in liver tissues. In addition, hepatic TG content and histopathological scores were significantly reduced. L. brevis MG5311 increased the protein expression of SIRT1, PPARα, SOD1, CAT, and GPx 1/2 in liver tissue, while inhibiting CYP2E1 and SREBP-1c. These results indicated that L. brevis MG5311 alleviated ethanol-induced liver injury by inhibiting hepatic oxidative stress and promoting lipid metabolism. Therefore, L. brevis MG5311 may be a useful probiotic candidate for ameliorating or preventing ALD.

Research Insights

Lactobacillus brevis SBC8803→Reduced Liver Injury
L. brevis MG5311 administration significantly reduced serum ALT, AST, and triglyceride (TG) levels in ethanol-fed mice... hepatic TG content and histopathological scores were significantly reduced.
Effect
Beneficial
Effect size
Moderate
Lactobacillus brevis SBC8803→Reduced Oxidative Stress
L. brevis MG5311 also decreased malondialdehyde levels and increased glutathione peroxidase (GPx) activity in liver tissues.
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus→Reduced Hepatic Oxidative Stress
L. brevis MG5311 also decreased malondialdehyde levels and increased glutathione peroxidase (GPx) activity in liver tissues... increased the protein expression of SIRT1, PPARα, SOD1, CAT, and GPx 1/2 in liver tissue, while inhibiting CYP2E1 and SREBP-1c.
Effect
Beneficial
Effect size
Moderate
Lactobacillus rhamnosus→Reduced Liver Injury
L. brevis MG5311 administration significantly reduced serum ALT, AST, and triglyceride (TG) levels in ethanol-fed mice... hepatic TG content and histopathological scores were significantly reduced.
Effect
Beneficial
Effect size
Moderate