CBT-BF3 (Probiotics, Postbiotics, and Cytosine-Phosphate-Guanine Oligodeoxynucleotide) on T Cell Differentiation and Gut Microbiota.

2024-11
Food science of animal resources 44(6)
- Gwang Il Kim
- Hwa Yeong Jeong
- In Sung Kim
- Seung Ho Lee
- Sung Hak Kim
- Yang Soo Moon
- Kwang Keun Cho

PubMed: 39554831
DOI: 10.5851/kosfa.2024.e100

The gut microbiota is an immune system regulator in the gut-skin axis. Dysfunctional interactions between the gut microbiota and the gut immune system can lead to the development of skin diseases such as atopic dermatitis (AD). Probiotics and postbiotics positively affect the balance of the gut microbiota, immune regulation, protection against pathogens, and barrier integrity. This study investigated the effects of probiotic Bifidobacterium bifidum, postbiotic B. bifidum (heat-killed), and cytosine-phosphate-guanine oligodeoxynucleotide (CpG ODN) on the gut microbiota and T cell differentiation in NC/Nga mice induced with AD. 2,4-Dinitrochlorobenzene-induced AD mice had an increased SCORing atopic dermatitis-index and increased mRNA expression levels of Th2 and Th17 cell transcription factors and cytokines, and thymic stromal lymphopoietin (TSLP) cytokine in their mesenteric lymph nodes (mLNs; p<0.05). However, oral administration of the three types of B. bifidum (probiotics, postbiotics, CpG ODN) to AD mice decreased the mRNA expression levels of Th2 and Th17 cell transcription factors and cytokines as well as TSLP cytokine. They increased the mRNA expression levels of regulatory T (Treg) cell transcription factor and cytokine, galectin-9, and filaggrin genes (p<0.05). These effects were more noticeable in the mLNs than in the spleen. In addition, AD mice showed a decrease in Faecalibacterium prausnitzii, Roseburia spp., Leuconostoc citreum, Weissella cibaria, and Weissella koreensis (p<0.05). However, oral administration of the three types of B. bifidum increased Bacteroides spp., Bifidobacterium spp., F. prausnitzii, and Roseburia spp. (p<0.05).

Research Insights

Supplement	Health Outcome	Effect Type	Effect Size
Bifidobacterium bifidum Bb-02	Improved Gut Microbiota Composition	Beneficial	Moderate
Bifidobacterium bifidum Bb-02	Improved Immune Function	Beneficial	Moderate
Bifidobacterium bifidum Bb-02	Reduced Atopic Dermatitis Severity	Beneficial	Moderate
Bifidobacterium bifidum BB-06	Improved Gut Microbiota Composition	Beneficial	Moderate
Bifidobacterium bifidum BB-06	Improved Immune Function	Beneficial	Moderate
Bifidobacterium bifidum BB-06	Reduced Atopic Dermatitis Severity	Beneficial	Moderate