VHPriobi O17.

2022-10
Heliyon 8(10)
- Shudong Peng
- Chaoqun Guo
- Songjie Wu
- Zhi Duan

PubMed: 36299523
DOI: 10.1016/j.heliyon.2022.e11125

The purpose of this study was to isolate exopolysaccharides (EPS) from lactic acid bacteria (LAB) and evaluate EPS anti-UVB viability. Lacticaseibacillus rhamnosus VHPriobi O17 with high EPS production was screened from 34 strains of LAB. The EPS (OP-2) produced by L. rhamnosus VHPriobi O17 was purified by alcohol precipitation and DEAE-μSphere anion exchange chromatography. By ion chromatography, FT-IR spectrum and gel column chromatography, EPS (OP-2) was a novel Man-like polysaccharide with the weight-averaged molecular of 84.2 kDa. The EPS (OP-2) can effectively alleviate HaCaT cells apoptosis and overproduction of reactive oxygen species (ROS) induced by UVB. The results also showed that it inhibited the release of pro-inflammatory cytokines (IL-1α, IL-6 and IL-8); and suppressed the phosphorylation cascade of JNK and p38 MAPK to reduce the expression level of active-caspase3, ultimately prevented cell apoptosis. Thus, the EPS produced by L. rhamnosus VHPriobi O17 have the potential to be used for human anti-UVB irradiation.

Research Insights

Supplement	Health Outcome	Effect Type	Effect Size
Lactobacillus rhamnosus lysate powder	Reduced Cell Apoptosis	Beneficial	Moderate
Lactobacillus rhamnosus lysate powder	Reduced Oxidative Stress	Beneficial	Moderate
Lactobacillus rhamnosus lysate powder	Reduced Pro-inflammatory Cytokine Release	Beneficial	Moderate
Lactobacillus rhamnosus lysate powder	Reduced UVB-Induced Skin Cell Damage	Beneficial	Moderate