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Isolation, characterization and anti-UVB irradiation activity of an extracellular polysaccharide produced by Lacticaseibacillus rhamnosus VHPriobi O17.

  • 2022-10
  • Heliyon 8(10)
    • Shudong Peng
    • Chaoqun Guo
    • Songjie Wu
    • Zhi Duan

Study Design

Population
HaCaT cells
Methods
EPS (OP-2) produced by Lacticaseibacillus rhamnosus VHPriobi O17 was purified by alcohol precipitation and DEAE-μSphere anion exchange chromatography, and its anti-UVB effects were evaluated in HaCaT cells
The purpose of this study was to isolate exopolysaccharides (EPS) from lactic acid bacteria (LAB) and evaluate EPS anti-UVB viability. Lacticaseibacillus rhamnosus VHPriobi O17 with high EPS production was screened from 34 strains of LAB. The EPS (OP-2) produced by L. rhamnosus VHPriobi O17 was purified by alcohol precipitation and DEAE-μSphere anion exchange chromatography. By ion chromatography, FT-IR spectrum and gel column chromatography, EPS (OP-2) was a novel Man-like polysaccharide with the weight-averaged molecular of 84.2 kDa. The EPS (OP-2) can effectively alleviate HaCaT cells apoptosis and overproduction of reactive oxygen species (ROS) induced by UVB. The results also showed that it inhibited the release of pro-inflammatory cytokines (IL-1α, IL-6 and IL-8); and suppressed the phosphorylation cascade of JNK and p38 MAPK to reduce the expression level of active-caspase3, ultimately prevented cell apoptosis. Thus, the EPS produced by L. rhamnosus VHPriobi O17 have the potential to be used for human anti-UVB irradiation.

Research Insights

  • Lactobacillus rhamnosus→Reduced Cell Apoptosis

    The EPS (OP-2) can effectively alleviate HaCaT cells apoptosis and overproduction of reactive oxygen species (ROS) induced by UVB... ultimately prevented cell apoptosis.

    Effect
    Beneficial
    Effect size
    Moderate
  • Lactobacillus rhamnosus→Reduced Oxidative Stress

    The EPS (OP-2) can effectively alleviate HaCaT cells apoptosis and overproduction of reactive oxygen species (ROS) induced by UVB.

    Effect
    Beneficial
    Effect size
    Moderate
  • Lactobacillus rhamnosus→Reduced Pro-inflammatory Cytokine Release

    It inhibited the release of pro-inflammatory cytokines (IL-1α, IL-6 and IL-8).

    Effect
    Beneficial
    Effect size
    Moderate
  • Lactobacillus rhamnosus→Reduced UVB-Induced Skin Cell Damage

    The EPS (OP-2) can effectively alleviate HaCaT cells apoptosis and overproduction of reactive oxygen species (ROS) induced by UVB... inhibited the release of pro-inflammatory cytokines (IL-1α, IL-6 and IL-8); and suppressed the phosphorylation cascade of JNK and p38 MAPK to reduce the expression level of active-caspase3, ultimately prevented cell apoptosis.

    Effect
    Beneficial
    Effect size
    Moderate
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