Lactobacillus gasseri-derived postbiotics promote osteogenic differentiation in BMSCs via ameliorating mitochondrial oxidative stress.

2026-01
Biochemical and biophysical research communications 795

PubMed: 41389616
DOI: 10.1016/j.bbrc.2025.153113

Study Design

Population: bone marrow mesenchymal stem cells (BMSCs); in vivo rat calvarial defects
Methods: The effects of postbiotics on BMSCs viability were assessed via MTT assay. Osteogenic differentiation was evaluated using RT-qPCR, ALP staining, Alizarin Red staining, and immunofluorescence. Antioxidant effects were examined by measuring ROS levels in H2O2-induced BMSCs. For in vivo analysis, postbiotics were incorporated into gelatin methacrylate (GelMA) and injected into rat calvarial defects, with bone regeneration assessed via micro-CT and histology analysis.

Animal Study

Objective

Craniomaxillofacial inflammatory bone defects, such as those in periodontitis, present significant regeneration challenges due to complex inflammatory microenvironments. Increasing attention has been directed toward the potential of probiotics and postbiotics in promoting osteogenesis and modulating inflammatory responses. This study aimed to elucidate the antioxidant and osteogenic effects of postbiotics from Lactobacillus gasseri ATCC 33323 on bone marrow mesenchymal stem cells (BMSCs).

Methods

The effects of postbiotics on BMSCs viability were assessed via MTT assay. Osteogenic differentiation was evaluated using RT-qPCR, ALP staining, Alizarin Red staining, and immunofluorescence. Antioxidant effects were examined by measuring ROS levels in H₂O₂-induced BMSCs. For in vivo analysis, postbiotics were incorporated into gelatin methacrylate (GelMA) and injected into rat calvarial defects, with bone regeneration assessed via micro-CT and histology analysis.

Results

Postbiotics (150 μg/mL) significantly enhanced BMSC proliferation, migration, and osteogenic differentiation. They increased mRNA expression of ALP, OPN, OSTERIX, and Col-1, elevated RUNX2 and OPN protein levels, and stimulated ALP activity and calcium salt deposition. Additionally, postbiotics markedly reduced intracellular ROS in H₂O₂-induced BMSCs. In vivo, postbiotic treatment significantly enhanced bone regeneration and reduced local inflammation in calvarial defects.

Conclusion

Postbiotics from Lactobacillus gasseri promote BMSC osteogenic differentiation by alleviating mitochondrial oxidative stress, demonstrating potential as a therapeutic strategy for bone regeneration under inflammatory conditions.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus gasseri	—	Improved Bone Regeneration	Beneficial	Moderate	View source Postbiotics (150 μg/mL) significantly enhanced BMSC proliferation, migration, and osteogenic differentiation... In vivo, postbiotic treatment significantly enhanced bone regeneration and reduced local inflammation in calvarial defects.
Lactobacillus gasseri	—	Reduced Inflammation	Beneficial	Small	View source In vivo, postbiotic treatment significantly enhanced bone regeneration and reduced local inflammation in calvarial defects.
Lactobacillus gasseri	—	Reduced Oxidative Stress	Beneficial	Moderate	View source Additionally, postbiotics markedly reduced intracellular ROS in H2O2-induced BMSCs.