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Microbiome-mediated colonization resistance to carbapenem-resistant Klebsiella pneumoniae in ICU patients.

  • 2025-08-09
  • NPJ biofilms and microbiomes 11(1)
    • Jing Yang
    • Yi Zhou
    • Aiping Du
    • Zhongwei Zhang
    • Bo Wang
    • Yongming Tian
    • Huan Liu
    • Lin Cai
    • Fang Pang
    • Yumei Li
    • Chunhua Du
    • Xijun Wu
    • Cong Yan
    • Wei Wu
    • Min Jiang
    • Ke Shen
    • Chi Zhang
    • Yu Feng
    • Yan Kang
    • Bairong Shen
    • Zhiyong Zong

Study Design

Type
Observational
Population
healthy individuals and ICU patients, distinguishing those with and without CRKP colonization
Methods
We analyzed the gut microbiome and metabolic profiles of healthy individuals and ICU patients; antibiotic-perturbed mouse with fecal microbiota transplantation (FMT); both in vitro and in vivo experiments; probiotic supplementation or FMT
Carbapenem-resistant Klebsiella pneumoniae (CRKP) causes serious intensive care unit (ICU)-acquired infections, yet the mechanisms of microbiota-mediated colonization resistance remain unclear. We analyzed the gut microbiome and metabolic profiles of healthy individuals and ICU patients, distinguishing those with and without CRKP colonization. ICU patients showed distinct microbial communities compared to healthy controls, and CRKP-positive patients exhibited unique microbial and metabolic signatures. We demonstrated that a healthy gut microbiome is essential for providing resistance against CRKP colonization in antibiotic-perturbed mouse with fecal microbiota transplantation (FMT). Both in vitro and in vivo experiments revealed that Lactiplantibacillus plantarum and Bifidobacterium longum as significant contributors to the decolonization of CRKP. Furthermore, we showed that probiotic supplementation or FMT significantly improved CRKP colonization resistance. The findings highlight that a specific gut microbiome is essential for resisting CRKP colonization, and that targeted microbiome restoration may serve as a viable strategy to prevent CRKP colonization in ICU patients.

Research Insights

  • Bifidobacterium plantarum→Improved Gut Microbial Persistence

    Both in vitro and in vivo experiments revealed that Lactiplantibacillus plantarum and Bifidobacterium longum as significant contributors to the decolonization of CRKP. Furthermore, we showed that probiotic supplementation or FMT significantly improved CRKP colonization resistance.

    Effect
    Beneficial
    Effect size
    Moderate
  • Bifidobacterium plantarum→Reduced CRKP Colonization

    Both in vitro and in vivo experiments revealed that Lactiplantibacillus plantarum and Bifidobacterium longum as significant contributors to the decolonization of CRKP. Furthermore, we showed that probiotic supplementation or FMT significantly improved CRKP colonization resistance.

    Effect
    Beneficial
    Effect size
    Moderate
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