- 2023-08-20
- Nutrients 15(16)
- Beate Obermüller
- Georg Singer
- Bernhard Kienesberger
- Barbara Mittl
- Vanessa Stadlbauer
- Angela Horvath
- Wolfram Miekisch
- Patricia Fuchs
- Martina Schweiger
- Laura Pajed
- Holger Till
- Christoph Castellani
Study Design
- Sample size
- n = 24
- Population
- 24 male BALB/c mice
- Methods
- gavage-fed with the probiotic formulation or water, once a day for 3 weeks; in the third week, the mice received intraperitoneal cyclophosphamide
- Rigorous Journal
- Animal Study
Cancer therapy is often associated with severe side effects such as drug induced weight loss, also known as chemotherapy-induced cachexia. The aim of this study was to investigate the effects of a multispecies probiotic (OMNi-BiOTiC® 10 AAD) in a chemotherapy mouse model. A total of 24 male BALB/c mice were gavage-fed with the probiotic formulation or water, once a day for 3 weeks. In the third week, the mice received intraperitoneal cyclophosphamide. At euthanasia, the organs were dissected, and serum was sampled for cytokine analysis. Tight junction components, myosin light chain kinase, mucins, and apoptosis markers were detected in the ileum and colon using histological analyses and qRT-PCR. Lipolysis was analyzed by enzymatic activity assay, Western blotting analyses, and qRT-PCR in WAT. The fecal microbiome was measured with 16S-rRNA gene sequencing from stool samples, and fecal volatile organic compounds analysis was performed using gas chromatography/mass spectrometry. The probiotic-fed mice exhibited significantly less body weight loss and adipose tissue wasting associated with a reduced CGI58 mediated lipolysis. They showed significantly fewer pro-inflammatory cytokines and lower gut permeability compared to animals fed without the probiotic. The colons of the probiotic-fed animals showed lower inflammation scores and less goblet cell loss. qRT-PCR revealed no differences in regards to tight junction components, mucins, or apoptosis markers. No differences in microbiome alpha diversity, but differences in beta diversity, were observed between the treatment groups. Taxonomic analysis showed that the probiotic group had a lower relative abundance of Odoribacter and Ruminococcus-UCG014 and a higher abundance of Desulfovibrio. VOC analysis yielded no significant differences. The results of this study indicate that oral administration of the multispecies probiotic OMNi-BiOTiC® 10 AAD could mitigate cyclophosphamide-induced chemotherapy side effects.
Research Insights
They showed significantly fewer pro-inflammatory cytokines and lower gut permeability compared to animals fed without the probiotic.
- Effect
- Beneficial
- Effect size
- Moderate
They showed significantly fewer pro-inflammatory cytokines and lower gut permeability compared to animals fed without the probiotic.
- Effect
- Beneficial
- Effect size
- Small
The probiotic-fed mice exhibited significantly less body weight loss and adipose tissue wasting associated with a reduced CGI58 mediated lipolysis.
- Effect
- Beneficial
- Effect size
- Moderate
The colons of the probiotic-fed animals showed lower inflammation scores and less goblet cell loss.
- Effect
- Beneficial
- Effect size
- Small
They showed significantly fewer pro-inflammatory cytokines and lower gut permeability compared to animals fed without the probiotic.
- Effect
- Beneficial
- Effect size
- Moderate
The probiotic-fed mice exhibited significantly less body weight loss and adipose tissue wasting associated with a reduced CGI58 mediated lipolysis.
- Effect
- Beneficial
- Effect size
- Moderate