Probiotics Upregulate Trefoil Factors and Downregulate Pepsinogen in the Mouse Stomach.

2019-08-10
International journal of molecular sciences 20(16)

PubMed: 31405107
DOI: 10.3390/ijms20163901

Study Design

Population: Weight-matching littermate male mice (n = 14)
Methods: The treated mice received VSL#3 (5 mg/day/mouse) by gastric gavage for 10 days. Control mice received only the vehicle. Food consumption and bodyweight were monitored. All mice were injected intraperitoneally with bromodeoxyuridine (120 mg/Kg bodyweight) two hours before sacrificed. Stomach tissues were processed for lectin- and immunohistochemical examination, and real-time quantitative polymerase chain reaction was used to provide relative changes in expression of gastric cell lineages specific genes.

Rigorous Journal
Animal Study

Probiotics are used in the management of some gastrointestinal diseases. However, little is known about their effects on normal gastric epithelial biology. The aim of this study was to explore how the probiotic mixture VSL#3 affects gastric cell lineages in mice with a special focus on protective and aggressive factors. Weight-matching littermate male mice (n = 14) were divided into treated and control pairs. The treated mice received VSL#3 (5 mg/day/mouse) by gastric gavage for 10 days. Control mice received only the vehicle. Food consumption and bodyweight were monitored. All mice were injected intraperitoneally with bromodeoxyuridine (120 mg/Kg bodyweight) two hours before sacrificed to label S-phase cells. Stomach tissues were processed for lectin- and immunohistochemical examination. ImageJ software was used to quantify immunolabeled gastric epithelial cells. Real-time quantitative polymerase chain reaction was used to provide relative changes in expression of gastric cell lineages specific genes. Results revealed that treated mice acquired (i) increased production of mucus, trefoil factor (TFF) 1 and TFF2, (ii) decreased production of pepsinogen, and (iii) increased ghrelin-secreting cells. No significant changes were observed in bodyweight, food consumption, cell proliferation, or parietal cells. Therefore, VSL#3 administration amplifies specific cell types specialized in the protection of the gastric epithelium.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus brevis SBC8803	—	Improved Gastric Mucosal Protection	Beneficial	Moderate	View source treated mice acquired (i) increased production of mucus, trefoil factor (TFF) 1 and TFF2, (ii) decreased production of pepsinogen, and (iii) increased ghrelin-secreting cells. ... Therefore, VSL#3 administration amplifies specific cell types specialized in the protection of the gastric epithelium.
Lactobacillus brevis SBC8803	—	Increased Mucin Production	Beneficial	Small	View source treated mice acquired (i) increased production of mucus, trefoil factor (TFF) 1 and TFF2
Lactobacillus brevis SBC8803	—	No Change in Body Weight or Food Consumption	Neutral	Small	View source No significant changes were observed in bodyweight, food consumption
Lactobacillus brevis SBC8803	—	Reduced Pepsinogen Production	Beneficial	Moderate	View source (ii) decreased production of pepsinogen