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Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier.

  • 2010-06
  • American Journal of Physiology-Gastrointestinal and Liver Physiology 298(6)
    • J. Karczewski
    • F. Troost
    • I. Konings
    • J. Dekker
    • M. Kleerebezem
    • R. Brummer
    • J. Wells

Abstract

Lactobacillus plantarum, a commensal bacterium of humans, has been proposed to enhance the intestinal barrier, which is compromised in a number of intestinal disorders. To study the effect of L. plantarum strain WCFS1 on human barrier function, healthy subjects were administered L. plantarum or placebo in the duodenum for 6 h by means of a feeding catheter. The scaffold protein zonula occludens (ZO)-1 and transmembrane protein occludin were found to be significantly increased in the vicinity of the tight-junction (TJ) structures, which form the paracellular seal between cells of the epithelium. In an in vitro model of the human epithelium, L. plantarum induced translocation of ZO-1 to the TJ region; however, the effects on occludin were minor compared with those seen in vivo. L. plantarum was shown to activate Toll-like receptor 2 (TLR2) signaling, and treatment of Caco-2 monolayers with the TLR2 agonist Pam(3)-Cys-SK4(PCSK) significantly increased fluorescent staining of occludin in the TJ. Pretreatment of Caco-2 monolayers with L. plantarum or PCSK significantly attenuated the effects of phorbol ester-induced dislocation of ZO-1 and occludin and the associated increase in epithelial permeability. Our results identifying commensal bacterial stimulation of TLR2 in the gut epithelium as a regulator of epithelial integrity have important implications for understanding probiotic mechanisms and the control of intestinal homeostasis.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus Bulgaricus & Lactobacillus PlantarumImproved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus Bulgaricus & Lactobacillus PlantarumIncreased Tight Junction Protein LevelsBeneficial
Moderate
Lactobacillus plantarumImproved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarumReduced Epithelial Barrier DisruptionBeneficial
Moderate
Lactobacillus plantarum 14DImproved Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum 14DImproved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum 14DIncreased Tight Junction Protein LevelsBeneficial
Moderate
Lactobacillus plantarum DSM 6595Activated TLR2 Signaling PathwayBeneficial
Moderate
Lactobacillus plantarum DSM 6595Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum DSM 6595Increased Tight Junction Protein LevelsBeneficial
Moderate
Lactobacillus plantarum DSM 6595Reduced Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum DSM 6596Improved Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum DSM 6596Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum DSM 6596Increased Tight Junction Protein LevelsBeneficial
Moderate
Lactobacillus plantarum HA-119Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum HA-119Reduced Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum KABP™-061Activated TLR2 Signaling PathwayBeneficial
Moderate
Lactobacillus plantarum KABP™-061Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum KABP™-061Reduced Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum L-137Activated TLR2 Signaling PathwayBeneficial
Moderate
Lactobacillus plantarum L-137Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum L-137Reduced Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum LP-01Activated TLR2 Signaling PathwayBeneficial
Moderate
Lactobacillus plantarum LP-01Improved Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum LP-01Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum LP-01Increased Tight Junction Protein LevelsBeneficial
Moderate
Lactobacillus plantarum N13Improved Tight Junction Protein LocalizationBeneficial
Moderate
Lactobacillus plantarum R1012Improved Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum R1012Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum Rosell-1012Improved Epithelial IntegrityBeneficial
Moderate
Lactobacillus plantarum Rosell-1012Improved Intestinal Epithelial Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum Rosell-1012Reduced Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum SD-5209Activated TLR2 Signaling PathwayBeneficial
Moderate
Lactobacillus plantarum SD-5209Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum SD-5209Reduced Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum SD5209Activated TLR2 Signaling PathwayBeneficial
Moderate
Lactobacillus plantarum SD5209Improved Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum SD5209Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum SD5209Increased Tight Junction Protein LevelsBeneficial
Moderate
Lactobacillus plantarum UALp-05Improved Epithelial Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum UALp-05Reduced Epithelial PermeabilityBeneficial
Moderate
Lactobacillus plantarum UALp-05MImproved Epithelial IntegrityBeneficial
Moderate
Lactobacillus plantarum UALp-05MImproved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus plantarum UALp-05MIncreased Tight Junction Protein LevelsBeneficial
Moderate
Lactobacillus plantarum WCFS1Improved Epithelial Barrier FunctionBeneficial
Moderate
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