Bifidobacterium animalis subsp. lactis BB-12 Has Effect Against Obesity by Regulating Gut Microbiota in Two Phases in Human Microbiota-Associated Rats
- 2022-01-10
- Frontiers in Nutrition 8
- Kemin Mao
- Jie Gao
- Xianghong Wang
- Xiyu Li
- S. Geng
- Tuo Zhang
- F. A. Sadiq
- Yaxin Sang
- PubMed: 35083265
- DOI: 10.3389/fnut.2021.811619
Abstract
Bifidobacterium animalis subsp. lactis BB-12 (BB-12) is an extensively studied probiotics species, which has been reported to improve the human gut microbiota. This study aimed to confirm the effects of BB-12 on high-fat diet (HFD)-induced gut microbiota disorders. The probiotic BB-12 was consumed by human microbiota-associated rats and changes in gut microbiota were compared using next generation sequencing of the fecal samples collected from the normal chow group, the HFD group, and the BB-12-supplemented group. The enterotypes switched from Prevotella dominant to Akkermansia dominant as a result of switching diet from normal chow to HFD. BB-12 conferred protection on the gut microbiota composition of the rats by increasing the abundance of Prevotella and decreasing the abundance of Clostridium, Blautia, and Bacteroides in 0-3 weeks. In addition, Prevotella-dominant enterotype was maintained, which provides improve obesity effects. A decrease in body weight and the Firmicutes/Bacteroidetes ratio were also observed at week 3. While in 4-8 weeks, the enrichment of short-chain fatty acids-producing bacteria such as Eubacterium and Parabacteroides and probiotics such as Bifidobacterium was observed. The results revealed that BB-12 against obesity by regulating gut microbiota in two phases. After a short-term intervention, BB-12 supplementation suppressed the transition from the healthy to obesity state by protecting Prevotella-dominant enterotype, whereas after a long-term intervention, BB-12 ameliorates obesity by enriching beneficial bacteria in the gut.
Keywords: Bifidobacterium animalis subsp. lactis BB-12; Prevotella; enterotypes; gut microbiota; obesity.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Bifidobacterium animalis | Improved Gut Microbiome Composition | Beneficial | Large |
| Bifidobacterium animalis | Increased Beneficial Gut Bacteria | Beneficial | Moderate |
| Bifidobacterium animalis | Maintained Prevotella-Dominant Gut Microbiota | Beneficial | Large |
| Bifidobacterium animalis | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium animalis | Reduced Obesity | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis | Increased Beneficial Gut Bacteria | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Increased Protective Fecal Bifidobacterial Diversity | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Reduced Obesity | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Regulated Microbiota Composition | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BB-12 | Increased Protective Fecal Bifidobacterial Diversity | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BB-12 | Maintained Gut Microbiota Composition | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BB-12 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BB-12 | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BB-12 | Suppressed Transition to Obesity | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Improved Gut Microbiome Composition | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis BLC1 | Increased Protective Fecal Bifidobacterial Diversity | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Maintained Prevotella-Dominant Gut Microbiota | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis BLC1 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Increased Protective Fecal Bifidobacterial Diversity | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Maintained Prevotella-Dominant Gut Microbiota | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis BS-01 | Optimized Gut Microbiota Composition | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis BS-01 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Improved Gut Microbiome Composition | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Improved Obesity Metrics | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Increased Beneficial Gut Bacteria | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Maintained Gut Microbiota Composition | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Maintained Prevotella-Dominant Gut Microbiota | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis VK2 | Increased Beneficial Gut Bacteria | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis VK2 | Maintained Prevotella-Dominant Gut Microbiota | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis VK2 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium bifidum BB-06 | Increased Beneficial Gut Bacteria | Beneficial | Moderate |
| Bifidobacterium bifidum BB-06 | Maintained Prevotella-Dominant Gut Microbiota | Beneficial | Large |
| Bifidobacterium bifidum BB-06 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium bifidum BB-06 | Reduced Gut Microbiota Disorder | Beneficial | Moderate |
| Bifidobacterium bifidum BB-06 | Reduced Obesity | Beneficial | Moderate |
| Bifidobacterium bifidum/lactis Bb-02 | Improved Abundance of Beneficial Bacteria | Beneficial | Large |
| Bifidobacterium bifidum/lactis Bb-02 | Prevention of Obesity Transition | Beneficial | Large |
| Bifidobacterium bifidum/lactis Bb-02 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium lactis | Improved Gut Microbiome Composition | Beneficial | Large |
| Bifidobacterium lactis | Increased Beneficial Gut Bacteria | Beneficial | Moderate |
| Bifidobacterium lactis | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Bifidobacterium lactis | Reduced Obesity | Beneficial | Moderate |
| Bifidobacterium lactis Bb-02 | Increased Protective Fecal Bifidobacterial Diversity | Beneficial | Moderate |
| Bifidobacterium lactis Bb-02 | Maintained Gut Microbiota Composition | Beneficial | Moderate |
| Bifidobacterium lactis Bb-02 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium lactis BB-12 | Improved Gut Microbiome Composition | Beneficial | Large |
| Bifidobacterium lactis BB-12 | Increased Protective Fecal Bifidobacterial Diversity | Beneficial | Moderate |
| Bifidobacterium lactis BB-12 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium lactis HDS Bb-02 | Altered Gut Microbiota Composition | Beneficial | Moderate |
| Bifidobacterium lactis HDS Bb-02 | Maintained Prevotella-Dominant Gut Microbiota | Beneficial | Large |
| Bifidobacterium lactis HDS Bb-02 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium lactis UABla-12 | Increased Production of Short-Chain Fatty Acids by Gut Bacteria | Beneficial | Large |
| Bifidobacterium lactis UABla-12 | Maintained Prevotella-Dominant Gut Microbiota | Beneficial | Large |
| Bifidobacterium lactis UABla-12 | Optimized Gut Microbiota Composition | Beneficial | Large |
| Bifidobacterium lactis UABla-12 | Reduced Body Weight | Beneficial | Moderate |
| Bifidobacterium lactis UABla-12 | Reduced Obesity | Beneficial | Moderate |
| Bifidobacterium lactis UABla-12 | Reduced Risk of Obesity | Beneficial | Moderate |