Hypoglycemic, Antioxidant Activities, and Probiotic Characteristics of Lacticaseibacillus rhamnosus LBUX2302 Isolated from Stool Samples of Neonates.
- 2025-05-18
- Life (Basel, Switzerland) 15(5)
- Pedro A Reyes-Castillo
- Ana Laura Esquivel-Campos
- Edgar Torres-Maravilla
- Eduardo Zúñiga-León
- Felipe Mendoza-Pérez
- Rosa González-Vázquez
- María Guadalupe Córdova-Espinoza
- María Angélica Gutiérrez-Nava
- Raquel González-Vázquez
- Lino Mayorga-Reyes
- PubMed: 40430230
- DOI: 10.3390/life15050804
Study Design
- Population
- healthy newborns
- Methods
- A novel Lacticaseibacillus rhamnosus strain was isolated and assessed for its hypoglycemic and antioxidative activity, along with other probiotic properties
Lacticaseibacillus species have shown potential in managing hyperglycemia, hypercholesterolemia, and oxidative stress, depending on the strain and species. This study aimed to isolate a novel Lacticaseibacillus rhamnosus strain from healthy newborns and assess its hypoglycemic and antioxidative activity, along with other probiotic properties. A non-hemolytic L. rhamnosus LBUX2302 was isolated, and it exhibited survival rates of 2.7%, 22%, and 27.5% at pH 2, 3, and 5 for 120 min. It metabolized various carbon sources and showed resistance to gentamicin, dicloxacillin, and penicillin; coaggregated with Salmonella typhi ATCC14028, Staphylococcus aureus STCC6538, and Escherichia coli O157:H7. L. rhamnosus LBUX2302 showed hydrophobicity, autoaggregation, and adhesion to HaCat, HeLa, MCF-7, SK-LU-1, and SW620 cell lines. It also exhibited extracellular activity of bile salt hydrolase. Enzymatic inhibition assays revealed 66% and 24% inhibitions of α-amylase and α-glucosidase, respectively. Its cell-free supernatant inhibited DPPH (89%), hydroxyl (81%), and superoxide anion radicals (61%). Also, antioxidant activity was observed in whole cells and cell fragments. Finally, the presence of ferulic acid activity was detected. The results highlight L. rhamnosus LBUX2302 as a promising probiotic with hypoglycemic and antioxidant effects, warranting further in vivo evaluation for its possible inclusion in functional food and health formulations.
Research Insights
The results highlight L. rhamnosus LBUX2302 as a promising probiotic with hypoglycemic and antioxidant effects, warranting further in vivo evaluation for its possible inclusion in functional food and health formulations.
- Effect
- Beneficial
- Effect size
- Moderate
Its cell-free supernatant inhibited DPPH (89%), hydroxyl (81%), and superoxide anion radicals (61%). Also, antioxidant activity was observed in whole cells and cell fragments.
- Effect
- Beneficial
- Effect size
- Large
Enzymatic inhibition assays revealed 66% and 24% inhibitions of α-amylase and α-glucosidase, respectively.
- Effect
- Beneficial
- Effect size
- Moderate